抗Paxillin (phospho Tyr118)抗体 | Anti-Paxillin (phospho Tyr118) antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	WB analysis of whole cell extracts (30 μg lysate) of COS-7 (Lane 1) treated with 100 uM Pervanadate for 1 hour (Lane 2), A431 (Lane 3) treated with 100 uM Pervanadate for 1 hour (Lane 4), HeLa (Lane 5) treated with 100 uM Pervanadate for 1 hour (Lane 6) using GTX24833 Paxillin (phospho Tyr118) antibody. Dilution : 1:500
	WB analysis of samples using GTX24833 Paxillin (phospho Tyr118) antibody. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 6) eliminates the signal further verifying that the antibody is phospho-specific.

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製品情報

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP, IHC
Reactivity	Human, Mouse, Rat, Pig, Monkey, Xenopus

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500
ICC/IF	Assay dependent
IHC-P	Assay dependent
IP	Assay dependent
IHC	Assay dependent

Not tested in other applications.

Calculated MW	65 kDa. ( Note )

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PROPERTIES

Form	Liquid
Buffer	PBS pH 7.3, 50% glycerol, 1mg/ml BSA, 0.05% sodium azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human paxillin that contains tyrosine 118.
Purification	Purified by antigen-affinity chromatography
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	paxillin
Cellular Localization	Cytoplasm, cytoskeleton
Background	This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445). [provided by RefSeq, Aug 2011]
Database	・ Gene ID: 5829 PXN ・ UniProt: P49023 PXN

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Paron I et al. PLoS One 2011; 6 (6) : e21684 Tenascin-C enhances pancreatic cancer cell growth and motility and affects cell adhesion through activation of the integrin pathway.	Application : WB
Armstrong T et al. Clin Cancer Res 2004; 10 (21) : 7427-37 Type I collagen promotes the malignant phenotype of pancreatic ductal adenocarcinoma.	Application : WB Reactivity : Human

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