抗FAK (phospho Tyr397)抗体 | Anti-FAK (phospho Tyr397) antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

WB analysis of samples using GTX24803 FAK (phospho Tyr397) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 2), a generic phosphotyrosine containing peptide(Lane 3), the phosphopeptide corresponding to FAK (phospho Tyr402) , or the phosphopeptide immunogen (Lane 5). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.

ICC/IF analysis of HeLa cells using GTX24803 FAK (phospho Tyr397) antibody. Green : Primary antibody Blue : cell nucleusFixation/Permeabilization : ice-cold 95% methanol

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製品情報

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-Fr, IP, ChIP assay
Reactivity	Human, Mouse, Rat, Zebrafish, Drosophila, Cat, Dog, Chicken, Amphibians, Rhesus Monkey, Xenopus, Fish

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	Assay dependent
ICC/IF	Assay dependent
IHC-Fr	Assay dependent
IP	Assay dependent
ChIP assay	Assay dependent

Not tested in other applications.

Calculated MW	119 kDa. ( Note )

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PROPERTIES

Form	Liquid
Buffer	PBS pH 7.4, 50% glycerol, 1mg/ml BSA, 0.05% sodium azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human FAK that contains tyrosine 397. The sequence is conserved in mouse, rat, chicken and frog.
Purification	Purified by antigen-affinity chromatography
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	protein tyrosine kinase 2 , FADK , FAK , FAK1 , FRNK , PPP1R71 , p125FAK , pp125FAK
Cellular Localization	Cytoplasm, cytoskeleton,Nucleus
Background	This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2017]
Database	・ Gene ID: 5747 PTK2 ・ UniProt: Q05397 PTK2

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Terasaki M et al. Oncol Rep 2018; (Epub) Glycine and succinic acid are effective indicators of the suppression of epithelial-mesenchymal transition by fucoxanthinol in colorectal cancer stem-like cells.
Liu CC et al. Nat Commun 2016; 7 : 11798 Suspension survival mediated by PP2A-STAT3-Col XVII determines tumour initiation and metastasis in cancer stem cells.	Application : WB Reactivity : Human
McGrail DJ et al. Sci Rep 2015; 5 : 9529 Alterations in ovarian cancer cell adhesion drive * resistance by increasing microtubule dynamics in a FAK-dependent manner.	Application : ICC/IF Reactivity : Human
McAndrews KM et al. Sci Rep 2015; 5 : 16941 Mesenchymal Stem Cells Induce Directional Migration of Invasive Breast Cancer Cells through TGF-β.	Application : ICC/IF Reactivity : Human
Hsu HY et al. Evid Based Complement Alternat Med 2012; 2012 : 819632 Antimigratory Effects of the Methanol Extract from Momordica charantia on Human Lung Adenocarcinoma CL1 Cells.	Application : WB Reactivity : Human

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