抗Rb (phospho Ser249)抗体 | Anti-Rb (phospho Ser249) antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

WB analysis of whole cell extracts (30 μg lysate) of Jurkat (Lane 1), Serum Starved Jurkat (Lane 2), Serum Starved for overnight following by serum release (Lane 3), and Jurkat treated for 15 min with 25 ng/ml of Calyculin A (lane 4) using GTX24788 Rb (phospho Ser249) antibody. Dilution : 1:500-1:2000

WB (peptide competition) analysis of Jurkat cells in high growth phase using GTX24788 Rb (phospho Ser249) antibody prior incubated with a generic phosphoserine-containing peptide (Lane 2), a generic phosphothreonine- containing peptide (Lane 3), the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 4), or the phosphopeptide immunogen (Lane 5). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.

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製品情報

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB
Reactivity	Human, Mouse

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:2000

Not tested in other applications.

Calculated MW	106 kDa. ( Note )

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PROPERTIES

Form	Liquid
Buffer	PBS pH 7.3, 1mg/ml BSA, 50% glycerol, 0.05% sodium azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human Rb that contains serine 249 and threonine 252 (based on Swiss Protein database, accession number P06400). The sequence is conserved in human, mouse and rat.
Purification	Purified by antigen-affinity chromatography
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	RB transcriptional corepressor 1 , OSRC , PPP1R130 , RB , p105-Rb , pRb , pp110
Cellular Localization	Nucleus
Background	The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008]
Database	・ Gene ID: 5925 RB1 ・ UniProt: P06400 RB1

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