抗Hsp90 alpha抗体 | Anti-Hsp90 alpha antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	IHC-P analysis of differentiated colon adenocarcinoma tissue from human colon tissue sections using GTX22928 Hsp90 alpha antibody. Left : without primary antibodyRight : with primary antibodyAntigen retrieval : 10mM sodium citrate (pH 6.0) buffer for 20 minutes at 95ºCDilution : 1:100
	IHC-P analysis of human tonsil tissue using GTX22928 Hsp90 alpha antibody. Left : Primary antibodyRight : Negative control without primary antibodyAntigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutesDilution : 1:200
	IP analysis of HeLa cell lysates using GTX22928 Hsp90 alpha antibody. GTX22928 was used as IP and IB antibodyIP reaction : 2μg antibody / 500μg lysateDilution : 1:1000
	IHC-P analysis of human kidney tissue using GTX22928 Hsp90 alpha antibody. Left : Primary antibodyRight : Negative control without primary antibodyAntigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutesDilution : 1:100
	ICC/IF analysis of HeLa cells and NIH3T3 cells using GTX22928 Hsp90 alpha antibody. Green : Primary antibodyBlue : NucleiRed : ActinFixation : FormalinPermeabilization : 0.1% Triton X-100 in TBS for 10 minutesDilution : 1:100 for at least 1 hour at room temperature
	WB analysis of 50 ug of the indicated whole cell lysates using GTX22928 Hsp90 alpha antibody. Hsp86 also known as Hsp90 alpha Dilution : 1:1000
	IHC-P analysis of human breast carcinoma tissue using GTX22928 Hsp90 alpha antibody. Left : Primary antibodyRight : Negative control without primary antibodyAntigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutesDilution : 1:50
	WB analysis of 2-fold serial dilutions of HeLa cell lysate(starting at 10 ug, per well) using GTX22928 Hsp90 alpha antibody. Dilution : 1:1000
	WB analysis of 2-fold serial dilutions of HeLa cell lysate(starting at 10 ug, per well) using GTX22928 Hsp90 alpha antibody. Dilution : 1:1000
	WB analysis of Hsp86 (Hsp90 alpha) and Hsp84 (Hsp90 beta) using GTX22928 Hsp90 alpha antibody. (left) or GTX79247 HSP90 beta antibody respectively
	WB analysis of A431 cell lysate (Lane 3~6) using GTX22928 Hsp90 alpha antibody. Lanes 1 and 2 are control samples of purified, recombinant Hsp86 (Hsp90 alpha) and Hsp84 (Hsp90 beta), respectively.Dilution : 1:2000

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製品情報

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP, IHC
Reactivity	Human, Mouse, Rat, Rabbit, Sheep, Primate

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500 - 1:2000
ICC/IF	1:50-1:200
IHC-P	5 μg/ml
IP	2 μg
IHC	Assay dependent

Not tested in other applications.

Calculated MW	85 kDa. ( Note )
Specificity/Sensitivity	This antibody does not detect HSP84.

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PROPERTIES

Form	Liquid
Buffer	PBS, 1mg/ml BSA, 0.05% sodium azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Mouse
Immunogen	Synthetic peptide corresponding to residues P(2) E E T Q T Q D Q P M(12) of mouse HSP86.
Purification	Purified by affinity chromatography
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	heat shock protein 90, alpha (cytosolic), class A member 1 , 86kDa , 89kDa , AL024080 , AL024147 , Hsp86-1 , Hsp89 , Hsp90 , Hspca , hsp4
Cellular Localization	Nucleus,Cytoplasm,Cell membrane
Background	Heat shock proteins (HSP) are expressed in response to various biological stresses, including heat. HSP90 is a 90 kDa protein that is induced under stress conditions, but is also one of the most abundant cellular proteins found under non-stress conditions. HSP90 has been found to be associated with a number of other intracellular proteins, including steroid receptors, actin, tubulin, Ah receptor, and some kinases. Studies have shown that murine HSP90 exists as two forms, HSP84 and HSP86, coded by related but separate genes, with 86% homologous amino acid sequences. These forms are analogous to the two forms of human HSP90, HSP89 beta and HSP89 alpha. In an unstressed mouse fibroblast, the basal level of HSP84 is found to be double that of HSP86. However, after heat shock, HSP86 shows a greater increase. Studies also suggest that upon cellular differentiation, the level of HSP86, but not HSP84, decreases. HSP84 and HSP86, which may be subject to estrogenic regulation, have been found as components of the non-DNA binding form of mouse glucocorticoid receptor, but dissociated from the transformed DNA-binding form.
Database	・ Gene ID: 15519 Hsp90aa1 ・ UniProt: P07901 Hsp90aa1

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