抗HIF1 beta [2B10]抗体 | Anti-HIF1 beta [2B10] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	ICC/IF analysis of NRK cells using GTX22771 HIF1 beta antibody [2B10]. Fixation : 4% paraformaldehydePermeabilization : 0.1% Triton X-100 in PBS for 15 minutesDilution : 40 μg/ml in staining buffer for 1 hour at room temperature
	ICC/IF analysis of U251 cells using GTX22771 HIF1 beta antibody [2B10]. Cells were probed without (right) or with(left) an antibody.Green : Primary antibodyBlue : NucleiRed : ActinFixation : formaldehydeDilution : 1:100 overnight at 4 ºC
	ICC/IF analysis of A2058 Cells using GTX22771 HIF1 beta antibody [2B10]. Cells were probed without (right) or with(left) an antibody.Green : Primary antibodyBlue : NucleiRed : ActinFixation : formaldehydeDilution : 1:100 overnight at 4 ºC
	ICC/IF analysis of HeLa cells using GTX22771 HIF1 beta antibody [2B10]. Cells were probed without (right) or with(left) an antibody.Green : Primary antibodyBlue : NucleiRed : ActinFixation : formaldehydeDilution : 1:20 overnight at 4ºC
	WB analysis of whole cell extracts (30 μg lysate) of HeLa (Lane 1), MCF-7 (Lane 2), NIH/3T3 (lane 3) and PC 12 (Lane 4) using GTX22771 HIF1 beta antibody [2B10]. Dilution : 1:500-3000

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	2B10
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, IP, Gel supershift assays, IHC
Reactivity	Human, Mouse, Rat, Xenopus, Fish, Primate

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:3000
ICC/IF	1:1,000
IHC-P	5 μg/ml
IP	Assay dependent
Gel supershift assays	Assay dependent
IHC	Assay dependent

Not tested in other applications.

Calculated MW	87 kDa. ( Note )

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PROPERTIES

Form	Liquid
Buffer	PBS, 1mg/ml BSA, 0.05% sodium azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Synthetic peptide corresponding to residues C N(771) S Y N N E E F P D L T M F P P F S E(789) of human ARNT.
Purification	Purified IgG
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	aryl hydrocarbon receptor nuclear translocator , HIF-1-beta , HIF-1beta , HIF1-beta , HIF1B , HIF1BETA , TANGO , bHLHe2
Cellular Localization	Nucleus
Background	This gene encodes a protein containing a basic helix-loop-helix domain and two characteristic PAS domains along with a PAC domain. The encoded protein binds to ligand-bound aryl hydrocarbon receptor and aids in the movement of this complex to the nucleus, where it promotes the expression of genes involved in xenobiotic metabolism. This protein is also a co-factor for transcriptional regulation by hypoxia-inducible factor 1. Chromosomal translocation of this locus with the ETV6 (ets variant 6) gene on chromosome 12 have been described in leukemias. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2013]
Database	・ Gene ID: 405 ARNT ・ UniProt: P27540 ARNT

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Lalevee S et al. Cell Death Dis 2014; 5:e1408 miR455 is linked to hypoxia signaling and is deregulated in preeclampsia.
Zhao Y et al. Front Aging Neurosci 2014; 6:10 Chronic cerebral hypoperfusion causes decrease of O-GlcNAcylation, hyperphosphorylation of tau and behavioral deficits in mice.	Application : WB Reactivity : Mouse
Lee SJ et al. Autophagy 2011; 7 (8):829-39 Beclin 1 deficiency is associated with increased hypoxia-induced angiogenesis.
Liu S et al. PLoS One 2011; 6 (4):e17904 Regulation of mouse small heat shock protein αb-crystallin gene by aryl hydrocarbon receptor.	Application : WB Reactivity : Mouse
Serra-Perez A et al. J Biol Chem 2010; 285 (24):18217-24 Extended ischemia prevents HIF1alpha degradation at reoxygenation by impairing prolyl-hydroxylation: role of Krebs cycle metabolites.	Application : WB Reactivity : Human

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