抗alpha Adaptin [AP6]抗体 | Anti-alpha Adaptin [AP6] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	ICC/IF analysis of U251 cells using GTX22730 alpha Adaptin antibody [AP6]. Cells were probed without (right) or with(left) an antibody.Green : Primary antibodyBlue : NucleiRed : ActinFixation : formaldehydeDilution : 1:20 overnight at 4ºC
	ICC/IF analysis of HeLa cells using GTX22730 alpha Adaptin antibody [AP6]. Panel e is a no primary antibody control.Green : Primary antibodyBlue : NucleiRed : ActinFixation : 4% paraformaldehydePermeabilization : 0.1% Triton X-100 for 10 minuteDilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature
	ICC/IF analysis of MCF-7 cells using GTX22730 alpha Adaptin antibody [AP6]. Cells were probed without (right) or with(left) an antibody.Green : Primary antibodyBlue : NucleiRed : ActinFixation : formaldehydeDilution : 1:20 overnight at 4ºC
	ICC/IF analysis of HeLa cells using GTX22730 alpha Adaptin antibody [AP6]. Cells were probed without (right) or with(left) an antibody.Green : Primary antibodyBlue : NucleiRed : ActinFixation : formaldehydeDilution : 1:20 overnight at 4ºC

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	AP6
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, FACS, Inhibition assay
Reactivity	Human, Mouse, Rat, Bovine, Hamster, Primate

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:200
ICC/IF	1-2 μg/ml
IHC-P	Assay dependent
FACS	1μg per 10⁶ cells
Inhibition assay	Assay dependent

Not tested in other applications.

Calculated MW	108 kDa. ( Note )
Specificity/Sensitivity	This antibody recognizes the products of both alpha-adaptin genes, alpha A and alpha C as well as an alternatively spliced isoform of alpha adaptin found in neurons.

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PROPERTIES

Form	Liquid
Buffer	PBS, 0.05% sodium azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Purified adaptors.
Purification	Purified IgG
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	adaptor related protein complex 2 subunit alpha 1 , ADTAA , AP2-ALPHA , CLAPA1
Cellular Localization	Cell membrane
Background	This gene encodes the alpha 1 adaptin subunit of the adaptor protein 2 (AP-2) complex found in clathrin coated vesicles. The AP-2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles which links clathrin to receptors in vesicles. Alternative splicing of this gene results in two transcript variants encoding two different isoforms. A third transcript variant has been described, but its full length nature has not been determined. [provided by RefSeq, Jul 2008]
Database	・ Gene ID: 160 AP2A1 ・ UniProt: O95782 AP2A1

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