抗Lactate Dehydrogenase抗体 | Anti-Lactate Dehydrogenase antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	GeneTex biotinylated anti-lactate dehydrogenase antibody was used to stain MeOH/Acetone-fixed HeLa cells by immunofluorescence.
	Western blot analysis of anti-Lactate Dehydrogenase antibody (1:1000 dilution) in HeLa cell extracts. Reactivity with LDH is observed in the cytoplasmic fraction (CF) and little to no reactivity in the nuclear fraction (NF). A single band of ~36 kDa corresponds to the expected molecular weight for the protein. Personal communication Kuldeep Patel.

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製品情報

Host	Goat
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IP, ELISA
Reactivity	Human, Rabbit, Guinea pig

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	Assay dependent
ICC/IF	Assay dependent
IP	Assay dependent
ELISA	Assay dependent

Not tested in other applications.

Calculated MW	37 kDa. ( Note )
Specificity/Sensitivity	Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Lactate Dehydrogenase [Rabbit Muscle].

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PROPERTIES

Form	Liquid
Buffer	0.02 M Potassium phosphate, 0.15 M sodium chloride, pH 7.2, containing 0.01% sodium azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species	Rabbit
Immunogen	Lactate Dehydrogenase [Rabbit Muscle]
Purification	IgG fraction
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	lactate dehydrogenase A , LDH-M
Cellular Localization	Cytoplasm
Background	The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. (provided by RefSeq)
Database	・ Gene ID: 100009107 LDHA ・ UniProt: P13491 LDHA

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Crawford RM et al. EMBO J 2002; 21 (15):3936-48 M-LDH serves as a sarcolemmal K(ATP) channel subunit essential for cell protection against ischemia.

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