抗CXCR4抗体 | Anti-CXCR4 antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	ICC/IF analysis of HeLa cells using GTX22074 CXCR4 antibody. Working concentration : 2 μg/ml
	WB analysis of HeLa cell lysate using GTX22074 CXCR4 antibody. Working concentration : 0.5 μg/ml
	ICC/IF analysis of HeLa cells using GTX22074 CXCR4 antibody. Working concentration : 20 μg/ml
	FACS analysis of HeLa cells using GTX22074 CXCR4 antibody. Working concentration : 0.1 μg/ml Purple: cells without staining Green: Isotype controlRed : CXCR4 antibody
	WB analysis of HeLa cells were transfected with control siRNAs (lane 1) or CXCR4 siRNAs (lane 2) using GTX22074 CXCR4 antibody. Loading : 10μg of lysates per laneDilution : 2 μg/ml
	WB analysis of various sample lysates using GTX22074 CXCR4 antibody. Loading : 15μg of lysates per laneDilution : 1 μg/ml
	WB analysis of CXCR4 partial recombinant protein using GTX22074 CXCR4 antibody. Dilution : 0.1 μg/ml
	WB analysis of various sample lysates using GTX22074 CXCR4 antibody. Loading : 20μg of lysates per laneDilution : 2 μg/ml
	IHC-P analysis of human spleen tissue using GTX22074 CXCR4 antibody. Antigen retrieval : Heat mediation with a citrate buffer (pH6)Dilution : 5 μg/ml

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製品情報

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA
Reactivity	Human, Mouse, Rat

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1 - 2 μg/mL
ICC/IF	10 μg/ml
IHC-P	Assay dependent
IHC-Fr	Assay dependent
FACS	0.1 μg/ml
IP	Assay dependent
ELISA	Assay dependent

Not tested in other applications.

Calculated MW	40 kDa. ( Note )
Positive Control	HeLa Cell Slide(GTX25529) , HeLa Cell Lysate
Specificity/Sensitivity	CXCR4 Antibody is predicted to not cross-react with other CXCR familiy members.

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PROPERTIES

Form	Liquid
Buffer	PBS with 0.02% sodium azide.
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Peptide sequence corresponding to amino acids 1-14 of human CD184.
Purification	Purified by antigen-affinity chromatography
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	C-X-C motif chemokine receptor 4 , CD184 , D2S201E , FB22 , HM89 , HSY3RR , LAP-3 , LAP3 , LCR1 , LESTR , NPY3R , NPYR , NPYRL , NPYY3R , WHIM , WHIMS
Cellular Localization	Cell membrane,Cell junction,Lysosome
Background	This gene encodes a CXC chemokine receptor specific for stromal cell-derived factor-1. The protein has 7 transmembrane regions and is located on the cell surface. It acts with the CD4 protein to support HIV entry into cells and is also highly expressed in breast cancer cells. Mutations in this gene have been associated with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
Database	・ Gene ID: 7852 CXCR4 ・ UniProt: P61073 CXCR4

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Feng H et al. Br J Cancer 2017; (Epub) ALDH1A3 affects colon cancer in vitro proliferation and invasion depending on CXCR4 status.
Zhao Z et al. Int J Cancer 2017; (Epub) Intraductal papillary mucinous neoplasm of the pancreas rapidly xenografts in chicken eggs and predicts aggressiveness.	Application : IHC-Fr Reactivity : Human
Zhang Y et al. Oncotarget 2015; 6 (12) : 9999-10015 aspirin counteracts cancer stem cell features, desmoplasia and * resistance in pancreatic cancer.	Application : IHC-Fr Reactivity : Human
Isayev O et al. Oncotarget 2014; 5 (13) : 5177-89 inhibition of glucose turnover by 3-bromopyruvate counteracts pancreatic cancer stem cell features and sensitizes cells to *.	Application : IHC-Fr Reactivity : Human
Jin UH et al. BMC Cancer 2014; 14 : 498 The aryl hydrocarbon receptor ligand omeprazole inhibits breast cancer cell invasion and metastasis.	Application : WB, IHC-P Reactivity : Human

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