抗BrdU抗体 | Anti-BrdU antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	Tissue culture cells were labelled prior to transplantation and then identified in in vivo tissue using GTX21893 sheep polyclonal BrdU antibody (10ug/ml incubated overnight at room temperature) with a TRITC conjugated secondary antibody. Image
	Immunoprecipitation using anti-BrdU antibody (GTX21893) at 25-100ug/ml

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製品情報

Host	Sheep
Clonality	Polyclonal
Isotype	IgG
Application	ICC/IF, IHC-P, IHC-Fr, IP, ELISA, IHC
Reactivity	Human

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APPLICATION

Application Note

ELISA: Use at an assay dependent dilution. IHC-P: Use at a dilution of 1/10. IHC-Fr: Use at a concentration of 10 μg/ml. IP: Use at a concentration of 25 - 100 μg/ml. Fixation in cold methanol for 30 minutes followed by immersion in 7 x 10^-3 M NaOH for 10-15 seconds allows BrdU staining with the simultaneous detection of nuclear cytoplasmic and membrane assigns as well as preservation of morphological detail. The unconjugated antibody was tested using immunoprecipitation against 5-Methyl Cytosine (5-MeC) and bromodeoxyuridine (BrdU) or control (no antigen) and assayed by A-405 spectrophotometry. At a concentration of 25 μg/mL, this product demonstrates 8 fold higher reactivity against BrdU than 5-MeC. Product titers out to 50 ng/mL in the purified form. For best results on unconjugated antibody, use the product at a concentration of 25 to 100ug/mL. Nearly complete immunoprecipitation was obtained at concentrations of 100 to 500ug/mL. The product has also been tested for utility for staining of bromodeoxyuridine incorporated into DNA of replicating cells. When utilized at a purified concentration of 10 μg/mL, the product is comparable to commercially available monoclonal antibodies commonly used for the same purpose. A key issue is that it is necessary to denature the DNA so as to expose the base to the antibodies. One protocol is: Sachiko Matsuuraa and Kazuo Suzukia Immunohistochemical Analysis of DNA Synthesis During Chronic Stimulation with Isoproterenol in Mouse Submandibular Gland . Journal of Histochemistry and Cytochemistry, Vol. 45, 1137-1146, Immunohistochemistry DNA-synthesizing cells were detected by peroxidase-anti-peroxidase (PAP) immunostaining with anti-BrdU antibodies by the method of Harms et al. 1986 , which was slightly modified as follows. Sections were treated with 2 N HCl at 60C for 15 min and washed in running water for 15 min. After dehydration in an ethanol series, the sections were etched with xylene (15 min, twice). Nuclear proteins, which mask incorporated BrdU, were digested with 0.025% protease (Type V; Sigma) in PBS at 37C for 10 min, and the sections were then washed with cold PBS three times for 5 min. Optimal dilutions/concentrations should be determined by the end user.

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PROPERTIES

Form	Liquid
Buffer	0.15M Phosphate-buffered saline, pH 7.5
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1.93 mg/ml (Please refer to the vial label for the specific concentration.)
Immunogen	Bromodeoxyuridine coupled to keyhole limpet hemocyanin (KLH).
Purification	Protein G affinity purified
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms

Bromodeoxyuridine antibody BUdr antibody

Background

The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information (such as the S-phase transit rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Besse HC et al. Cancers (Basel) 2019; 11 (10) Tumor Drug Distribution after Local Drug Delivery by Hyperthermia, In Vivo.	Application : IHC Reactivity : Human
Yang Y et al. Neurochem Res 2019; 44 (4) : 811-828 MiR-124 Enriched Exosomes Promoted the M2 Polarization of Microglia and Enhanced Hippocampus Neurogenesis After Traumatic Brain Injury by Inhibiting TLR4 Pathway.	Application : IHC-P
Piao JM et al. Cell Physiol Biochem 2018; 46 (3) : 890-906 MicroRNA-381 Favors Repair of Nerve Injury Through Regulation of the SDF-1/CXCR4 Signaling Pathway via LRRC4 in Acute Cerebral Ischemia after Cerebral Lymphatic Blockage.	Application : IHC-Fr
Hester MS et al. Brain Res 2018; (Epub) Intrauterine Inflammation Reduces Postnatal Neurogenesis in the Hippocampal Subgranular Zone and Leads to Accumulation of Hilar Ectopic Granule Cells.	Application : IHC
Ye Y et al. Stem Cells Int 2017; 2017 : 5841814 Electroacupuncture Improved Hippocampal Neurogenesis following Traumatic Brain Injury in Mice through Inhibition of TLR4 Signaling Pathway.

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