抗TGF beta [TB21]抗体 | Anti-TGF beta [TB21] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

WB analysis of platelet derived TGF beta at 12mg/lane (lane 2), 6mg/lane (lane 3), 3mg/lane (lane 4) and 1mg/mL (lane 5) with 4-chlorol-napthol as substrate using TGF beta antibody [TB21] at a dilution of 2mg/ml. Lane 1 is molecular weight markers.

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	TB21
Isotype	IgG1
Application	WB, IHC-P, IHC-Fr, FACS, ELISA, IHC, Neutralizing/Blocking
Reactivity	Human, Mouse, Rat, Rabbit, Sheep, Bovine, Mink, Mustelid

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APPLICATION

Application Note

Neutralizing:This antibody neutralizes TGF-beta activity in vitro, in an inhibition assay of CCL/64 cell growth and neutralizes the growth promoting action of TGF-beta in the NRK-49F colony forming assay. The effect of microinjection of this antibody into one blastomere of two cell stage Xenopus embryos indicated that it was able to neutralize effectively the bioactivity of TGF-beta in vivo. IHC:This antibody has been used to demonstrate TGF-beta in ovine ovarian tissue and human breast carcinoma at a dilution of 1:1000. As a consequence of the intense staining of the erythrocytes it is possible to locate a single cell within the ovarian stroma making it useful in locating very fine capillary networks within tissue. Western Blotting:This antibody when used at an antibody concentration of 5-20 ng/mL will allow visualization of 100 ng/lane of TGF-beta.

Positive Control	Ovine ovarian tissue, breast cancer sections
Specificity/Sensitivity	Western blotting demonstrated that this antibody reacts with the dimeric (25 Kd) and monomeric (12.5 kDa.) forms of TGF-beta under both non-reducing and reducing conditions respectively.

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PROPERTIES

Form	Liquid
Buffer	Preservative: NoneConstituents: 0.01M PBS. pH 7.0
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	TGF-beta 1 from human platelets
Purification	IgG fraction Protein G purified.
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Background	TGF beta (Transforming Growth Factor beta) is a multifunctional peptide that controls proliferation, differentiation and other functions in many cell types.

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Grabias BM et al. Am J Physiol Renal Physiol 2013; 305 (1) : F123-33 Notch4-dependent antagonism of canonical TGF-β1 signaling defines unique temporal fluctuations of SMAD3 activity in sheared proximal tubular epithelial cells.	Application : WB Reactivity : Human
Lee E et al. Cancer 2013; 119 (9) : 1752-60 Cohypermethylation of p14 in combination with CADM1 or DCC as a recurrence-related prognostic indicator in stage I esophageal squamous cell carcinoma.	Application : IHC-P Reactivity : Human
Jung Y et al. Int J Oncol 2012; 40 (4) : 1122-32 Identification of prognostic biomarkers for glioblastomas using protein expression profiling.
Cayli S et al. Acta Histochem 2012; 114 (1) : 43441 Jab1 expression is associated with TGF-β1 signaling in chronic rhinosinusitis and nasal polyposis.	Application : IHC Reactivity : Human
Hu HY et al. Int J Clin Exp Pathol 2016; 9 (6):6171-6178 ATRA alleviated endometrial fibrosis in a rabbit intrauterine adhesions model through downregulation of the TGF-β1/smad4 signaling pathway.	Application : WB, IHC-P Reactivity : Rabbit

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