抗Ki67抗体 | Anti-Ki67 antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	Immunohistochemical analysis of formalin-fixed paraffin-embedded human tonsil using Ki-67 antibody. Signal is detected using ABC method with AEC chromogen
	HT-29 xenograft tumor in mouse 20x pretreatment w/ citrate pH 6.0 using anti-Ki67 antibody GTX20833 at 5 ug/mL for 30 min at RT.
	This picture was kindly supplied as part of the review submitted by Dr Robert O'Donogue. Tissue sections from mouse lung were stained with GTX20833 at a dilution of 1/50.

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製品情報

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC
Reactivity	Human, Mouse, Rat, Zebrafish

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APPLICATION

Application Note

For IHC-P: Use at a concentration of 1 0 - 2 0 μg / m l f o r 3 0 m i n a t R T. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW	359 kDa. ( Note )
Positive Control	Tonsil or Breast carcinoma

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PROPERTIES

Form	Liquid
Buffer	10mM Phosphate-buffered saline containing 0.2% BSA and 0.1% sodium azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Synthetic peptide from the human Ki-67 allantigen.
Purification	Protein A affinity purified
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	Marker Of Proliferation Ki-67 , Kia , Mib- , Mib-1 , Ppp1R105 , Mki67
Cellular Localization	Nuclear
Background	Ki-67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67-positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki67 index and histologically low-grade tumours is strong.
Database	・ Gene ID: 4288 ・ UniProt: P46013

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Li M et al. Cell Death Dis 2020; 11 (1) : 31 The nuclear translocation of transketolase inhibits the farnesoid receptor expression by promoting the binding of HDAC3 to FXR promoter in hepatocellular carcinoma cell lines.	Application : WB Reactivity : Human
Bordignon P et al. Cell Rep 2019; 28 (9) : 2358-2372.e6 Dualism of FGF and TGF-帣 Signaling in Heterogeneous Cancer-Associated Fibroblast Activation with ETV1 as a Critical Determinant.	Application : ICC/IF Reactivity : Human
Chou CM et al. J Biomed Sci 2015; 22 : 102 Activation of MEK2 is sufficient to induce skin papilloma formation in transgenic zebrafish.	Application : IHC-P Reactivity : Zebrafish
Trazzi S et al. J Biol Chem 2010; 285 (13) : 10098-109 CB1 cannabinoid receptors increase neuronal precursor proliferation through AKT/glycogen synthase kinase-3beta/beta-catenin signaling.	Application : ICC/IF Reactivity : Mouse

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