抗Vimentin [J144]抗体 | Anti-Vimentin [J144] antibody

• 特長
• 価格
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	J144
Isotype	IgM
Application	WB, IHC
Reactivity	Human

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APPLICATION

Application Note

IHC and WB: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW	54 kDa. ( Note )
Specificity/Sensitivity	Detects the intermediate filament protein vimentin. The antibody gives a positive reaction with connective tissue, endothelial cells, vascular smooth muscle cells and astrocytes.

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PROPERTIES

Form	Liquid
Buffer	Phosphate buffered saline
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Human rhabdosarcoma cell line JR1.
Purification	Purified IgM
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	Vimentin , Vim
Cellular Localization	Cytoplasmic
Background	Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of malignant tumors, generally used with a panel of other antibodies including those recognising cytokeratins, lymphoid markers, S100, desmin and neurofilaments.
Database	・ Gene ID: 7431 ・ UniProt: P08670

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