抗FITC抗体 | Anti-FITC antibody

• 特長
• 価格
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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製品情報

Host	Goat
Clonality	Polyclonal
Isotype	IgG F(ab')2
Application	WB, ICC/IF, IHC-P, IHC-Fr, ELISA, IHC

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:200 - 1:1000
ICC/IF	Assay dependent
IHC-P	Assay dependent
IHC-Fr	Assay dependent
ELISA	1:2000 - 1:8000
IHC	Assay dependent

Not tested in other applications.

Specificity/Sensitivity	No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).

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PROPERTIES

Form	Liquid
Buffer	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Immunogen	Fluorescein conjugated to Goat IgG
Purification	Purified by antigen-affinity chromatography. Immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Background

FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Uchida T et al. Ren Fail 2017; 39 (1) : 340-349 Renoprotective effects of a dipeptidyl peptidase 4 inhibitor in a mouse model of progressive renal fibrosis.	Application : IHC-P
Gotanda Y et al. J Stroke Cerebrovasc Dis 2014; Efficient Transduction of 11 Poly-arginine Peptide in an Ischemic Lesion of Mouse Brain.	Application : ICC/IF, IHC-Fr

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