抗N-Cadherin [8C11]抗体 | Anti-N-Cadherin [8C11] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	WB analysis of 25 μg of various lysates using GTX19348 N-Cadherin antibody [8C11]. Dilution : 1:1000
	IHC-P analysis of human breast tissue using GTX19348 N-Cadherin antibody [8C11]. A : isotype controlB : Primary antibodyC : Primary antibody with magnified sectionAntigen retrieval : HEIR with a buffer (pH 6.2)Dilution : 2 μg/ml
	IP analysis of SH-SY5Y cell lysates using GTX19348 N-Cadherin antibody [8C11]. GTX19348 was used as IP and IB antibody IP reaction : 5μg antibody / 300μg lysate WB antibody dilution : 1:1000
	WB analysis of tissue lysate and whole cell extract (30 ug) of Mouse Brain (Lane 1) and MCF-7 (Lane 2) using GTX19348 N-Cadherin antibody [8C11]. Dilution : 1:1000
	WB analysis of 25 ug of Hela (Lane 1), HUVEC (Lane 2), and C2C12 cell lysates (Lane 3) using GTX19348 N-Cadherin antibody [8C11]. Dilution : 1:1000 (Hela) and 1:500 (HUVEC and C2C12)
	ICC/IF analysis of SH-SY5Y cells using GTX19348 N-Cadherin antibody [8C11]. Cells were probed without (left) or with(right) an antibody.Green : Primary antibodyBlue : NucleiRed : ActinFixation : FormalinPermeabilization : 0.1% Triton X-100 in TBS for 10 minutesDilution : 1:100 for at least 1 hour at room temperature
	IHC-P analysis of human heart tissue using GTX19348 N-Cadherin antibody [8C11]. Right : Primary antibodyLeft : Negative control without primary antibodyAntigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 minDilution : 1:20

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	8C11
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human, Mouse, Rabbit, Hamster, Avian

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:1000
ICC/IF	1:50-1:200
IHC-P	1:20
IP	5 μg

Not tested in other applications.

Calculated MW	100 kDa. ( Note )
Specificity/Sensitivity	This antibody is not suitable for frozen tissues in immunohistochemistry applications.

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PROPERTIES

Form	Liquid
Buffer	PBS, 1mg/ml BSA, 0.05% sodium azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Bacterial fusion protein corresponding to the extracellular domain of N-cadherin.
Purification	Protein G purified
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	cadherin 2 , CD325 , CDHN , CDw325 , NCAD
Cellular Localization	Cell membrane,Cell junction
Background	This gene encodes a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone. [provided by RefSeq, Nov 2015]
Database	・ Gene ID: 1000 CDH2 ・ UniProt: P19022 CDH2

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