抗TNF alpha [CH8820]抗体 | Anti-TNF alpha [CH8820] antibody

• 特長
• 価格
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	CH8820
Isotype	IgG1
Application	ELISA, IHC, Neutralizing/Blocking
Reactivity	Human

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APPLICATION

Application Note

ELISA: Use at an assay dependent dilution. GTX18696 can be used as a capture antibody in sandwich ELISA applications for human TNF-alpha detection in combination with a monoclonal tracer/detection antibody (biotinconjugated). 4 pg/ml of TNF-alpha in serum/plasma or medium can be detected with an assay range of 0 to 2000 pg/ml. Suggested capture coating dose: 1.0-2.0μg/well IHC: Use at a concentration of 10 μg/ml. Reacts with formalin-fixed paraffin-embedded tissues at a monoclonal antibody concentration of 10μg/mL. Strong reaction with normal kidney, liver with cirrhosis, and normal lung samples, positive reaction with lung cancer samples.Neut: GTX18696 neutralizes TNF-alpha activity in vitro. Use 2-5 ng of GTX18696 per μg of MAb. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW	26 kDa. ( Note )
Specificity/Sensitivity	This monoclonal antibody recognizes both recombinant and native human TNF-alpha.

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PROPERTIES

Form	Liquid
Buffer	Preservative: None. Constituents: 0.01M PBS. pH 7.2
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Recombinant full length protein (Human).
Purification	Protein G purified
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	Tumor Necrosis Factor , Dif , Tnf-Alpha , Tnfa , Tnfsf2 , Tnlg1F , Tnf
Cellular Localization	Secreted. Type 2 membrane protein or soluble form derived from the membrane by proteolytic cleavage.
Background	Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS PAGE under non reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.
Database	・ Gene ID: 7124 ・ UniProt: P01375

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