抗alpha Actinin 1 [0.T.02]抗体 | Anti-alpha Actinin 1 [0.T.02] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

WB analysis of A431 cell lysate using GTX18061 alpha Actinin 1 antibody [0.T.02].

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	0.T.02
Isotype	IgG1
Application	WB, ICC/IF, IHC-Fr
Reactivity	Human, Mouse, Rat, Xenopus

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	0.5-2μg/ml
ICC/IF	15μg/ml (fixed with methanol)
IHC-Fr	15μg/ml

Not tested in other applications.

Calculated MW	103 kDa. ( Note )
Specificity/Sensitivity	Recognizes human alpha-Actinin 1 isoform (non-muscle) at 100kD.

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PROPERTIES

Form	Liquid
Buffer	0.02M PBS, 0.25M NaCl, 0.1% sodium azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Human alpha-Actinin
Purification	Protein A purified
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	ACTN1,BDPLT15,actinin alpha 1,alpha Actinin 1
Cellular Localization	Cytoplasm,Cytoskeleton,Myofibril,Sarcomere,Z line,Cell membrane,Cell junction,Cell projection,Ruffle
Background	Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Database	・ Gene ID: 87 ACTN1 ・ UniProt: P12814 ACTN1

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Has C et al. Am J Pathol 2009; 175 (4) : 1442-52 Kindlin-1 Is required for RhoGTPase-mediated lamellipodia formation in keratinocytes.	Application : WB Reactivity : Human

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