抗alpha Actinin 4 [7H6]抗体 | Anti-alpha Actinin 4 [7H6] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	IHC-P analysis of human colon carcinoma tissue using GTX15648 alpha Actinin 4 antibody [7H6]. Left : Primary antibodyRight : Negative control without primary antibodyAntigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutesDilution : 1:200
	IHC-P analysis of human kidney tissue using GTX15648 alpha Actinin 4 antibody [7H6]. Left : Primary antibodyRight : Negative control without primary antibodyAntigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutesDilution : 1:200
	IHC-P analysis of human tonsil tissue using GTX15648 alpha Actinin 4 antibody [7H6]. Left : Primary antibodyRight : Negative control without primary antibodyAntigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutesDilution : 1:200
	WB analysis of samples using GTX15648 alpha Actinin 4 antibody [7H6].

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	7H6
Isotype	IgG
Application	WB, ICC/IF, IHC-P, FACS, ELISA
Reactivity	Human, Mouse

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	2 μg/mL
ICC/IF	4 μg/mL
IHC-P	4 μg/ml
FACS	1μg per 10⁶ cells
ELISA	Assay dependent

Not tested in other applications.

Calculated MW	105 kDa. ( Note )

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PROPERTIES

Form	Liquid
Buffer	PBS, 1mg/ml BSA, 0.05% sodium azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Synthetic peptide corresponding to residues A(884) P Y Q G P D A V P G A L D(897) of human alpha Actinin 4.
Purification	Protein G purified
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	actinin alpha 4 , ACTININ-4 , FSGS , FSGS1
Cellular Localization	Nucleus,Cytoplasm,Cell junction
Background	Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. [provided by RefSeq, Jul 2008]
Database	・ Gene ID: 81 ACTN4 ・ UniProt: O43707 ACTN4

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Durcin M et al. J Extracell Vesicles 2017; 6 (1) : 1305677 Characterisation of adipocyte-derived extracellular vesicle subtypes identifies distinct protein and lipid signatures for large and small extracellular vesicles.
Durcin M et al. JEV 2017; (Epub) Characterisation of adipocyte-derived extracellular vesicle subtypes identifies distinct protein and lipid signatures for large and small extracellular vesicles.	Application : WB Reactivity : Mouse

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