抗Carbonic Anhydrase IX抗体 | Anti-Carbonic Anhydrase IX antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	WB analysis of (1)HeLa, (2)MDA-MB-231 and (3)A549 cell lysate using GTX15086 Carbonic Anhydrase IX antibody.
	IHC-P analysis of human breast carcinoma tissue using GTX15086 Carbonic Anhydrase IX antibody. Dilution : 1:1000
	IHC-P analysis of renal carcinoma tissue using GTX15086 Carbonic Anhydrase IX antibody.
	IHC-Fr analysis of renal carcinoma tissue using GTX15086 Carbonic Anhydrase IX antibody. Panel A : primary antibodyPanel B : staining with normal rabbit serum
	WB analysis of rat renal cortex tissue lysate using GTX15086 Carbonic Anhydrase IX antibody.
	ICC/IF analysis of HEK 293 cells overexpressing Carbonic Anhydrase IX (Panel A) and mock cells (Panel B) using GTX15086 Carbonic Anhydrase IX antibody.
	WB analysis of HeLa cell lysate using GTX15086 Carbonic Anhydrase IX antibody.
	WB analysis of HEK overexpressing Carbonic Anhydrase IX cell lysate using GTX15086 Carbonic Anhydrase IX antibody. Rabbit IgG was used as a negative control.

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製品情報

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC-Fr, FACS, ELISA, ChIP assay, Gel supershift assays
Reactivity	Human, Mouse, Rat, Dog

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:2000
ICC/IF	1:1000
IHC-P	1:1000
IHC-Fr	1:1000
FACS	Assay dependent
ELISA	Assay dependent
ChIP assay	1:10 - 1:500
Gel supershift assays	Assay dependent

Not tested in other applications.

Calculated MW	50 kDa. ( Note )

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PROPERTIES

Form	Liquid
Buffer	PBS, 0.025% Sodium Azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	A synthetic peptide made to a C-terminal sequence of human Carbonic Anhydrase IX (within residues 400-459) [UniProt Q16790]
Purification	Purified by antigen-affinity chromatography
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	carbonic anhydrase 9 , CAIX , MN
Cellular Localization	Nucleus,Cell membrane,Nucleolus
Background	Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq]
Database	・ Gene ID: 768 CA9 ・ UniProt: Q16790 CA9

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Veelken C et al. Methods 2017; (Epub) Single cell-based automated quantification of therapy responses of invasive cancer spheroids in organotypic 3D culture.	Application : ICC/IF Reactivity : Human

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