抗Aurora A [35C1]抗体 | Anti-Aurora A [35C1] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

WB analysis of Aurora A kinase recombinant protein using GTX13824 Aurora A antibody

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	35C1
Isotype	IgG2b
Application	WB, ICC/IF, IHC-P, IP, IHC, PLA
Reactivity	Human, Mouse

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1/500-1/1,000
ICC/IF	Assay dependent
IHC-P	Assay dependent
IP	Assay dependent
IHC	Assay dependent
PLA	Assay dependent
Note : WB This antibody detects a band of approximately 46kDa in human HeLa and mouse M-ICc12 cell lysates.

Not tested in other applications.

Calculated MW	46 kDa. ( Note )
Specificity/Sensitivity	This antibody recognizes an epitope within the non-catalytic N-terminal domain of Aurora A. Clone 35C1 does not inhibit Aurora A kinase activity (Cremet et al. 2003).

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PROPERTIES

Form	Liquid
Buffer	PBS, 0.09% Sodium Azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1.0 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Recombinant Aurora-A
Purification	Protein G purified From tissue culture supernatant
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	Aurora Kinase A , Aik , Ark1 , Aura , Btak , Ppp1R47 , Stk15 , Stk6 , Stk7 , Aurka
Background	The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Database	・ Gene ID: 6790 ・ UniProt: O14965

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Lai CH et al. Cancer Lett 2020; 472 : 97-107 A selective Aurora-A 5'-UTR siRNA inhibits tumor growth and metastasis.	Application : WB Reactivity : Human
Wang YC et al. Exp Mol Med 2018; 50 (6) : 70 The EGF/hnRNP Q1 axis is involved in tumorigenesis via the regulation of cell cycle-related genes.	Application : IHC-P Reactivity : Human
Richards MW et al. Proc Natl Acad Sci U S A 2016; (Epub) Structural basis of N-Myc binding by Aurora-A and its destabilization by kinase inhibitors.	Application : PLA Reactivity : Human
Aradottir M et al. J Pathol Clin Res 2015; 1 (1) : 33-40 Aurora A is a prognostic marker for breast cancer arising in BRCA2 mutation carriers.	Application : IHC-P Reactivity : Human
Wen QJ et al. Nat Med 2015; 21 (12) : 1473-80 Targeting megakaryocytic-induced fibrosis in myeloproliferative neoplasms by AURKA inhibition.	Application : WB Reactivity : Mouse

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