抗Cytochrome C [7H8.2C12]抗体 | Anti-Cytochrome C [7H8.2C12] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	WB analysis of HeLa cell lysate using GTX13575 Cytochrome C antibody [7H8.2C12]. Dilution : 0.1μg/ml
	WB analysis of HeLa cell lysate using GTX13575 Cytochrome C antibody [7H8.2C12]. Dilution : 1:1000
	FACS (Intracellular staining) analysis of HeLa cells using GTX13575 Cytochrome C antibody [7H8.2C12]. Blue : Primary antibody Orange : isotype controlDilution : 10 μg/mLFixation : 4% PFAPermibilization : 0.1% saponin
	ICC/IF analysis of Hela cells using GTX13575 Cytochrome C antibody [7H8.2C12]. Red : primary antibody Blue : DAPI Dilution : 25 μg/mL
	IHC-P analysis of human heart tissue using GTX13575 Cytochrome C antibody [7H8.2C12]. Dilution : 1:200

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	7H8.2C12
Isotype	IgG2b
Application	WB, ICC/IF, IHC-P, FACS
Reactivity	Human, Mouse, Rat, Rabbit, Drosophila, Dog, Pig, Horse

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	0.05-0.5 μg/ml
ICC/IF	1:10 - 1:500
IHC-P	1:10 - 1:500
FACS	1:10 - 1:1000

Not tested in other applications.

Calculated MW	12 kDa. ( Note )
Specificity/Sensitivity	This antibody recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).

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PROPERTIES

Form	Liquid
Buffer	PBS, 0.5% BSA, 0.05% Sodium Azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Pig
Immunogen	Synthetic peptides corresponding to amino acids 1-80, 81-104 and 66-104 of pigeon CYT were used as the immunogen (Jemmerson et al. 1991). The antibody recognizes an epitope within amino acids 93-104 of pigeon cytochrome C based on competitive ELISA results (Jemmerson et al. 1991).
Purification	Protein G purified
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	cytochrome c, somatic , CYC , HCS , THC4
Background	This gene encodes cytochrome c, a component of the electron transport chain in mitochondria. The heme group of cytochrome c accepts electrons from the b-c1 complex and transfers electrons to the cytochrome oxidase complex. Cytochrome c is also involved in initiation of apoptosis. Upon release of cytochrome c to the cytoplasm, the protein binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Many cytochrome c pseudogenes exist, scattered throughout the human genome. [provided by RefSeq]
Database	・ Gene ID: 54205 CYCS ・ UniProt: P99999 CYCS

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Wu TH et al. Acta Neuropathol 2013; 125 (5) : 711-25 Loss of vesicular * release precedes tauopathy in degenerative dopaminergic neurons in a Drosophila model expressing human tau.	Application : WB Reactivity : Drosophila
Giri? M et al. J Surg Res 2010; 164 (2) : 266-75 Heme oxygenase-1 prevents hyperthyroidism induced hepatic damage via an antioxidant and antiapoptotic pathway.	Application : WB Reactivity : Rat

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