抗Hsp90 [16F1]抗体 | Anti-Hsp90 [16F1] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	Western blot analysis of HSP90: Lane 1: MW marker, Lane 2: HSP90 native protein, Lane 3: HSP90b recombinant protein, Lane 4: HSP90alpha recombinant protein, Lane 5: HeLa , Lane 6: L-929 , Lane 7: Rat-2 , Lane 8:RK-13.
	Immunohistochemistry (paraffin sections) analysis of human spleen tissue stained with HSP90, mAb (16F1) at 10μg/ml.

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製品情報

Host	Rat
Clonality	Monoclonal
Clone Name	16F1
Isotype	IgG2a
Application	WB, ICC/IF, IHC-P, IP, EIA, IHC
Reactivity	Human, Mouse, Rat, Sheep, Drosophila, Bovine, Dog, Hamster, Guinea pig, Pig, Monkey, Carp, Rainbow trout

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APPLICATION

Application Note

IHC-P: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. Detects both free and complexed Hsp90 and also picks up both hsp90 alpha and beta equally well (detects 100ng of each). WB: Use at a concentration of 2 μg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 83.2 kDa (beta) & 84.5 kDa (alpha)). Optimal dilutions/concentrations should be determined by the end user.

Positive Control	Heat-shocked HeLa cell lysate and Hsp90 protein
Specificity/Sensitivity	Detects both the alpha and beta isoforms of Hsp90

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PROPERTIES

Form	Liquid
Buffer	PBS, 0.1mM PMSF, 50% Glycerol containing no preservatives
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Human Hsp90 purified from therapeutic orchiectomy specimens.
Purification	Protein G affinity purified
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	FLJ31884 heat shock 90kDa protein 1 alpha Heat shock protein HSP 90 alpha HSP86 Hsp89 HSP90A HSPC1 HSPCAL1 HSPN LAP2 Lipopolysaccharide associated protein2 LPS associated protein 2
Cellular Localization	Cytoplasmic
Background	The 90kDa molecular chaperone family comprises several proteins including the 90kDa heat shock protein, Hsp90 and the 94kDa glucose regulated protein, grp94 which are major molecular chaperones of the cytosol and of the endoplasmic reticulum. In mammalian cells there are at least two Hsp90 isoforms, Hsp90a and hsp90s which are encoded by separate genes. The amino acid sequence of human and yeast Hsp90a is 85% and 90% homologous to that of Hsp90s respectively. All known members of the Hsp90 protein family are highly conserved, especially in the N terminal and C terminal regions which have been shown to contain independent chaperone sites with different substrate specificity. These ubiquitous and highly conserved proteins account for 1-2% of all cellular proteins in most cells. Hsp90 is part of the cell's powerful network of chaperones to fight the deleterious consequences of protein unfolding caused by nonphysiological conditions. However, in the absence of stress, Hsp90 is a necessary component of fundamental cellular processes such as hormone signaling and cell cycle control. In this context several key regulatory proteins such as steriod receptors, cell cycle kinases involved in signal transduction and p53 have been identified as substrates of Hsp90. It has been suggested that Hsp90 acts as a capacitor for morphological evolution by buffering widespread variation, which may affect morphogenic pathways.

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Espallergues J et al. J Neurosci 2012; 32 (13) : 4400-16 HDAC6 regulates glucocorticoid receptor signaling in serotonin pathways with critical impact on stress resilience.	Application : WB

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