抗Histone H3K9ac (Acetyl Lys9)抗体 | Anti-Histone H3K9ac (Acetyl Lys9) antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	IHC-P analysis of mouse liver tissue using GTX12178 Histone H3K9ac (acetyl Lys9) antibody at 0.1 μg/mL.
	IHC-P analysis of rat liver tissue using GTX12178 Histone H3K9ac (acetyl Lys9) antibody at 0.1 μg/mL.
	IHC-P analysis of mouse testis tissue using GTX12178 Histone H3K9ac (acetyl Lys9) antibody at 0.1 μg/mL.
	IHC-P analysis of rat lung tissue using GTX12178 Histone H3K9ac (acetyl Lys9) antibody at 0.1 μg/mL.
	WB analysis of NIH3T3 cells using GTX12178 Histone H3K9ac (acetyl Lys9) antibody. Lane 1 : Anti Histone H3K9ac (acetyl Lys9) antibody Lane 2 : Anti Histone H3K9ac (acetyl Lys9) + histone H3 (acetylated Lys9) peptide Lane 3 : Anti Histone H3K9ac (acetyl Lys9) + histone H3 peptide(non-acetylated)

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製品情報

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat, Drosophila, Bovine, Chicken, Caenorhabditis elegans, Frog

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:1,000
ICC/IF	1:1,000
IHC-P	Assay dependent
Note : WB 20-40 μg using RIPA extracts (0.45 mg) of rat PC12 cells

Not tested in other applications.

Calculated MW	15 kDa. ( Note )
Positive Control	NIH3T3 treated with sodium butyrate

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PROPERTIES

Form	Liquid
Buffer	0.01M PBS pH7.4, 15 mM sodium azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	2-2.5 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	synthetic acetylated [Ac-Lys9] histone H3 peptide (amino acids 7-20) corresponding to the N-terminus of human histone H3. The sequence is identical in many species including mouse, rat, bovine, chicken, frog, Drosophila, and C. elegans, and is highly conserved (single amino acid substitution) in Tetrahymena histone H3.
Purification	Purified by affinity chromatography
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	H3 histone family member 3A , H3.3A , H3F3
Cellular Localization	Nucleus
Background	Histone proteins H3, H4, H2A, and H2B function as building blocks to package eukaryotic DNA into repeating nucleosome units that are folded in higher order chromatin fibers. The nucleosome is composed of an octamer containing a H3/H4 tetramer and two H2A/H2B dimers, surrounded by approximately 146 base pairs of DNA. A diverse and elaborate array of post-translational modifications including acetylation, phosphorylation, methylation, ubiquitination, and ADP-ribosylation occurs on the N-terminal tail domains of histones. Acetylation of lysine residues within these N-terminal domains by histone acetyl-transferases (HATs), including Gcn5p, P/CAF, p300/CBP, and TAFII250, is associated with transcriptional activation. This modification results in remodeling of the nucleosome structure into an open conformation more accessible to transcription complexes. Conversely, histone deacetylation by histone deacetylases (HDACs) is associated with transcription repression reversing the chromatin remodeling process. In most species, histone H3 is primarily acetylated at lysine 9, 14, 18, and 23. Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms.
Database	・ Gene ID: 3020 H3F3A ・ UniProt: P84243 H3F3A

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Liang DY et al. Mol Pain 2014; 10 : 59 Epigenetic regulation of spinal cord gene expression controls opioid-induced hyperalgesia.	Application : IHC-P Reactivity : Mouse
Ishiguro K et al. J Immunol 2011; 186 (5) : 295907 Cutting edge: tubulin α functions as an adaptor in NFAT-importin β interaction.	Application : WB Reactivity : Human

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