抗S100 beta [SH-B4]抗体 | Anti-S100 beta [SH-B4] antibody
掲載日情報:2020/04/02 現在Webページ番号:122091
GeneTex社の抗S100 beta [SH-B4]抗体 | Anti-S100 beta [SH-B4] antibodyです。
※ 本製品は研究用です。研究用以外には使用できません。
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特長
- 高品質の抗体です。
- 幅広い研究分野に関連する抗体を取り揃えています。
- 使用されたアプリケーションや動物種などの情報が充実しています。
- 多数の論文で使用実績がある信頼性の高い抗体です。
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価格
[在庫・価格 :2025年04月26日 20時55分現在]
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Anti-S100β, Mouse-Mono(SH-B4) |
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Anti-S100β, Mouse-Mono(SH-B4) |
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[在庫・価格 :2025年04月26日 20時55分現在]
Anti-S100β, Mouse-Mono(SH-B4)
文献数: 6
- 商品コード:GTX11179
- メーカー:GNT
- 包装:100μl
- 価格:¥103,000
- 在庫:1個
- 納期:2~3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | レビューあり。 別名:S100 calcium binding protein B,NEF,S100,S100-B,S100beta クローン:SH-B4 Genbank No: 6285 |
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法規制等 | |||
保存条件 | -20℃ | 法規備考 | |
抗原種 | Bovine | 免疫動物 | Mouse |
交差性 | Bovine/Cat/Dog/Goat/Human/Mouse/Pig/Rabbit/Rat/Sheep | 適用 | ELISA,IC,IF,IHC |
標識 | Unlabeled | 性状 | Purified |
吸収処理 | クラス | IgG | |
クロナリティ | Monoclonal | フォーマット | |
掲載カタログ |
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製品記事 | 抗S100β(S100B)抗体(GeneTex社) |
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関連記事 | GeneTex社における抗体の品質管理 |
Anti-S100β, Mouse-Mono(SH-B4)
文献数: 6
- 商品コード:GTX11179
- メーカー:GNT
- 包装:100μl
- 価格:¥103,000
- 在庫:1個
- 納期:2~3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | レビューあり。 別名:S100 calcium binding protein B,NEF,S100,S100-B,S100beta クローン:SH-B4 Genbank No: 6285 |
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法規制等 | |||
保存条件 | -20℃ | 法規備考 | |
抗原種 | Bovine | 免疫動物 | Mouse |
交差性 | Bovine/Cat/Dog/Goat/Human/Mouse/Pig/Rabbit/Rat/Sheep | 適用 | ELISA,IC,IF,IHC |
標識 | Unlabeled | 性状 | Purified |
吸収処理 | クラス | IgG | |
クロナリティ | Monoclonal | フォーマット | |
掲載カタログ |
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製品記事 | 抗S100β(S100B)抗体(GeneTex社) |
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関連記事 | GeneTex社における抗体の品質管理 |
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製品情報
Host | Mouse |
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Clonality | Monoclonal |
Clone Name | SH-B4 |
Isotype | IgG1 |
Application | ICC/IF, IHC-P, IHC-Fr, ELISA |
Reactivity | Human, Rat, Rabbit, Goat, Sheep, Bovine, Cat, Dog, Pig |
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APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.Application | Dilution |
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ICC/IF | Assay dependent |
IHC-P | 1:100 |
IHC-Fr | Assay dependent |
ELISA | Assay dependent |
Calculated MW | 11 kDa. ( Note ) |
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Positive Control | human tongue |
Specificity/Sensitivity | Recognizes an epitope located on the β chain (i.e. in S-100a and S-100b) but not on the α chain of S-100 (i.e. in S-100a and S-100ao). In ELISA, recognition of S-100 subunit by clone SH-B4 is Ca2+-dependent. The product does not react with other members of the EF-hand family such as, calmodulin, parvalbumin, intestinal calcium-binding protein and myosin light chain. In immunohistochemistry, the antibody detects normal and neoplastic S-100 β subunit-containing cells (e.g. Schwann cells, chondrocytes, melanocytes, and melanotic tumors) in protease-digested, formalin-fixed, paraffin-embedded tissues. |
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PROPERTIES
Form | Liquid |
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Buffer | Ascites, 15 mM sodium azide |
Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
Antigen Species | Bovine |
Immunogen | bovine brain S-100b |
Purification | Unpurified |
Conjugation | Unconjugated |
Note | For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. |
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TARGET
Synonyms | S100 calcium binding protein B , NEF , S100 , S100-B , S100beta |
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Cellular Localization | Cytoplasm,Nucleus |
Background | S100 is a set of small, thermolabile, highly acidic dimer proteins, of approx. 20 kDa, widely distributed in different tissues. Dimeric combinations of two chains; the alpha chain (93 a.a. 10.4 kDa) and the beta chain (91 a.a. 10.5 kDa), form the three known subtypes of S100 [S100ao (alpha alpha), S100a (alpha beta) and S100b (beta beta). Although there is slight variation in the primary structure in different species, the S100 molecule is markedly conserved in the amino acid sequence, and the protein extracted from different organs of the same species is identical. The alpha and beta chains are 58% homologous (54 a.a.) and both have divalent cation binding sites situated toward the carboxy terminus and apparently similar functional features. S100 can be grouped with other calcium binding proteins, to which it has a significant sequence homology, particularly around the calcium binding domain, such as calmodulin, parvalbumin, intestinal calcium binding protein, myosin light chain, and troponin-C. Hence, S100 is a calcium modulated protein that binds calcium and zinc ions reversibly at physiologic pH and ionic strength, followed by a conformational change in the molecule. S100 is considered to be a cell-growth regulator, but other functions have been suggested, e.g., increasing the membrane permeability to cations under physiologic conditions, stimulation of nucleolar RNA polymerase activity, interaction with the tumor suppressor protein p53 and as a carrier of proteins and free fatty acids in adipocytes. Human S100 containing cells are subdivided to three groups, S100b containing cells (such as Schwann cells, pituicytes of the neurohypophysis, Langerhanis cells, and interdigitating cells), S100a containing cells (such as glial cells and melanocytes) and S100ao containing cells (such as neurons, ganglion cells, slow skeletal muscle cells, cardiac cells, monocytes and some macrophages). Although the tissue distribution of S100 is too broad to conform to a single histogenetic pattern, it is sufficiently restricted that the localization of this protein is useful in the differential diagnosis of neoplasms and proliferative processes. Monoclonal antibody reacting specifically with the beta subunit of S100 may be use to distinguish malignant melanoma from undifferentiated carcinoma or lymphoma, and to distinguish leiomyomas from schwannomas and their counterparts in the gastrointestinal tract. |
Database | ・ Gene ID: 6285 S100B ・ UniProt: P04271 S100B |
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REFERENCEE(抜粋)
Application Reference | Application/Reactivity |
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Mikawa S et al. Neuroscience 2014; 258 : 16-33 Chordin expression in the adult rat brain. | Application : IHC-Fr Reactivity : Rat |
Sango K et al. J Neurosci Res 2011; 89 (6) : 898-908 Spontaneously immortalized Schwann cells from adult Fischer rat as a valuable tool for exploring neuron-Schwann cell interactions. | Application : ICC/IF Reactivity : Rat |
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