IL-27 組換え体(リコンビナント)タンパク質 | Recombinant Mouse IL-27

• 2025/12/01

  試薬 特別価格

  [RSD]厳選 抗体＆リコンビナントタンパク質 30％OFFキャンペーン[～2026/02/27] NEW

  期間：2025/12/01 ～2026/02/27

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  R&D Systems, Inc.（RSD）

• バルク注文に関して
• CF(Carrier-Free)とは？
• Product Details
• Additional Information
• Citations

バルク注文に関して

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CF(Carrier-Free)とは？

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Product Details

Source

Mouse myeloma cell line, NS0-derived

Accession

O35228 (EBI3) & Q8K3I6 (p28)

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Predicted Molecular Mass

48.7 kDa

SDS-PAGE

60-65 kDa, reducing conditions

Activity

Measured in an anti-viral assay using HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. Bender H. et al. (2009) Hepatology 50

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Additional Information

Molecule Information

IL-27

Aliases

undefined

Entrez Gene IDs

246778 (Human); 246779 (Mouse)

Background

IL-27

Interleukin-27 (IL-27) is a heterodimeric group 2 receptor ligand molecule that belongs to the IL-6/IL-12 family of long type I cytokines. It is composed of EBI3 (EBV-induced gene 3), a 34 kDa glycoprotein that is related to the p40 subunit of IL-12 and IL-23, and p28, the28 kDa glycoprotein that is related to the p35 chain of IL-12). The human EBI3 gene encodes a 229 amino acid(aa) precursor that contains a 20 aa signal peptide and 209 aa mature protein. The mature region contains two potential N-linkedglycosylation sites, two fibronectin type III domains, and two pairs of conserved cysteine residues with a WSxWS-like motif thatplaces the IL-27 molecule in the hematopoietin receptor family.Although p40, the EBI3 counterpart in IL-12, is known to formhomodimers, there is no evidence to date that EBI3 also homodimerizes.Human EBI3 is 61% aa identical to mouse EBI3. The human p28 gene encodes a 243 aa precursor that contains a 28 aa signal sequence and 215 aa mature region. The mature region is characterized by the presence of four α-helices, placing it in the IL-6 family of helical cytokines. Human p28 is 74% aa identical to mouse p28. IL-27 is expressed by monocytes, endothelial cells and dendritic cells. IL-27 binds to and signals through a heterodimeric receptor complex composed of WSX-1 (TCCR) and gp130. Evidence suggests IL-27 interacts only with WSX-1. IL-27 has both anti- and pro-inflammatory properties. As an anti-inflammatory, IL-27 seems to induce a general negative feedback program that limits T and NK-T cell activity. At the onset of infection, IL-27 induces an IL-12 receptor on naïve CD4+ T cells, making them susceptible to subsequent IL-12 activity (and possible Th1 development).

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Citations

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