IL-3 組換え体(リコンビナント)タンパク質 | Recombinant Human IL-3

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• CF(Carrier-Free)とは？
• Product Details
• Additional Information
• Citations

バルク注文に関して

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CF(Carrier-Free)とは？

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Product Details

Source

E. coli-derived, Ala20-Phe152, with and without an N-terminal Met

Accession

Q6GS87

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Predicted Molecular Mass

15 kDa

Activity

Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140

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Additional Information

Molecule Information

IL-3

Aliases

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Entrez Gene IDs

3562 (Human); 16187 (Mouse); 24495 (Rat)

Background

IL-3

Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. In addition, IL-3 also affects the functional activity of mature mast cells, basophils, eosinophils and macrophages. Because of its multiple functions and targets, it was originally studied under different names, including mast cell growth factor P-cell stimulating factor, burst promoting activity, multi-colony stimulating factor, thy-1 inducing factor and WEHI-3 growth factor. In addition to activated T cells, other cell types such as human thymic epithelial cells, activated mouse mast cells, mouse keratinocytes and neurons/astrocytes can also produce IL-3. At the amino acid sequence level, mature human and mouse IL-3 share only 29% sequence identity. Consistent with this lack of homology, IL-3 activity is highly species-specific and human IL-3 does not show activity on mouse cells. IL-3 exerts its biological activities through binding to specific cell surface receptors. The high affinity receptor responsible for IL-3 signaling is composed of alpha and beta subunits. The IL-3 R alpha is a member of the cytokine receptor super family and binds IL-3 with low affinity. Two distinct beta subunits, AIC2A (betaIL-3) and AIC2B (betac) are present in mouse cells. BetaIL-3 also binds IL-3 with low affinity and forms a high affinity receptor with the alpha subunit. The betac subunit does not bind any cytokine but forms functional high affinity receptors with the alpha subunit of the IL-3, IL-5 and GM-CSF receptors. Receptors for IL-3 are present on bone marrow progenitors, macrophages, mast cells, eosinophils, megakaryocytes, basophils and various myeloid leukemic cells.

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Citations

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