IL-23 組換え体(リコンビナント)タンパク質 | Recombinant Human IL-23

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• CF(Carrier-Free)とは？
• Product Details
• Additional Information
• Citations

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CF(Carrier-Free)とは？

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Product Details

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived

Accession

P29460 (p40) & AAG37232 (p19)

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Predicted Molecular Mass

55.0 kDa (IL-23 single chain); 36.5 kDa (p40) & 18.5 kDa (p19)

SDS-PAGE

65 kDa, 43-45 kDa and 20 kDa, reducing conditions

Activity

Measured by its ability to induce IL-17 secretion by mouse splenocytes. Aggarwal, S. et al. (2003) J. Biol. Chem. 278

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Additional Information

Molecule Information

IL-23

Aliases

IL23A; SGRF

Entrez Gene IDs

51561 (Human); 83430 (Mouse)

Background

IL-23

Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12. The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11. The p40 subunit is homologous to the extracellular domains of the hematopoietic cytokine receptors. Mouse p19 cDNA encodes a 196 amino acid residue (aa) precursor protein with a putative 19 aa signal peptide and 177 aa mature protein. Human and mouse p19 share 70% aa sequence identity. Although p19 is expressed by activated macrophages, dendritic cells, T cells, and endothelial cells, only activated macrophages and dendritic cells express p40 concurrently to produce IL-23. The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R). IL-23 has biological activities that are similar to, but distinct from IL-12. Both IL-12 and IL-23 induce proliferation and IFN-gamma production by human T cells. While IL-12 acts on both naive and memory human T cells, the effects of IL-23 is restricted to memory T cells. In mouse, IL-23 but not IL-12, has also been shown to induce memory T cells to secret IL-17, a potent proinflammatory cytokine. IL-12 and IL-23 can induce IL-12 production from mouse splenic DC of both the CD8- and CD8+ subtypes, however only IL-23 can act directly on CD8+ DC to mediate immunogenic presentation of poorly immunogenic tumor/self peptide.

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Citations

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