Human IL-1 beta/IL-1F2 QuantiGlo ELISA Kit
掲載日情報：2016/08/18 現在Webページ番号：111382
R&D Systems製のHuman IL-1 beta/IL-1F2を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月26日 00時00分現在]
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 5.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL), Citrate Plasma (100 µL)
- Sensitivity
- 0.55 pg/mL
- Assay Range
- 1.4 - 1,000 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, Citrate Plasma, EDTA Plasma)
- Specificity
- Natural and recombinant human IL-1 beta. This assay also recognizes natural and recombinant rhesus macaque IL-1 beta.
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The QuantiGlo Human IL-1 beta Chemiluminescent Immunoassay is a 5.5 hour solid phase ELISA designed to measure human IL-1 beta in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-1 beta and antibodies raised against the recombinant protein. It has been shown to accurately quantitate the recombinant factor. Results obtained using natural IL-1 beta showed linear curves that were parallel to the standard curves obtained using the QuantiGlo kit standards. These results indicate that this kit can be used to determine relative mass values for natural IL-1 beta. Reports indicate that this and other ELISA kits calibrated using mature IL-1 beta as a standard will detect, but considerably underestimate, the unprocessed IL-1 beta precursor present in samples. In biological samples other than cell lysates, the precursor form of IL-1 beta is usually not the predominant form of IL-1 beta present and, additionally, is not biologically active. Therefore, results obtained using this kit should provide a useful measure of the levels of active IL-1 beta present in biological fluids.
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Precision
Intra-Assay Precision (Precision within an assay)
Four samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Four samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||||
| Sample | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 |
| n | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 7.96 | 103 | 638 | 4.18 | 9.86 | 136 | 695 | 5.13 |
| Standard Deviation | 0.31 | 5 | 30 | 0.16 | 0.71 | 9 | 38 | 0.4 |
| CV% | 3.9 | 4.9 | 4.7 | 3.8 | 7.2 | 6.6 | 5.5 | 7.8 |
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Recovery
The recovery of IL-1 beta spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 106 | 100-115 |
| Serum (n=4) | 96 | 87-102 |
| EDTA Plasma (n=4) | 94 | 88-107 |
| Heparin Plasma (n=4) | 93 | 87-103 |
| Citrate Plasma (n=4) | 95 | 90-101 |
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Linearity
To assess the linearity of the assay, four samples containing or spiked with high concentrations of IL-1 beta in various matrices were diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- IL-1 beta/IL-1F2
- Aliases
- IL1B; IL-1F2
- Entrez Gene IDs
- 3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 100034237 (Equine); 100135556 (Guinea Pig)
- Background
- IL-1 beta/IL-1F2
- IL-1 is a name that designates two proteins, IL-1 alpha and IL-1 beta, that are the products of distinct genes, but recognize the same cell surface receptors. IL-1 alpha and IL-1 beta are structurally related polypeptides that show approximately 25% homology at the amino acid level. Both proteins are produced by a wide variety of cells in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Calcium sensing receptor activation elevates proinflammatory factor expression in human adipose cells and adipose tissue.
Acevedo I, Ben-Jonathan N, Cifuentes M, Fuentes C, Hugo E, Reyes M, Tobar N, Villalobos E
Mol Cell Endocrinol 2012 361:24-30
Biological and technical variables affecting immunoassay recovery of cytokines from human serum and simulated vaginal fluid: a multicenter study.
Alfano M, Belec L, Carbonneil C, Chen S, Cosentino L, Cummins JE, Curtis K, Dezzutti CS, Donaghay M, Doncel GF, Donoval B, Fichorova RN, Grivel JC, Guzman E, Hayes M, Herold B, Hillier S, Lackman-Smith C, Landay A, Margolis L, Mayer KH, Pallansch-Cokonis M, Pasicznyk JM, Poli G, Reichelderfer P, Richardson-Harman N, Roberts P, Rodriguez I, Saidi H, Sassi RR, Shattock R
Anal. Chem. 2008 80:4741-51
Species: Human
Sample Type: WHO 86/680 reference standard
P2X receptor characterization and IL-1/IL-1Ra release from human endothelial cells.
Crossman DC, Dower SK, Francis SE, Holland KL, Stokes L, Surprenant A, Varcoe RW, Wilson HL
Br. J. Pharmacol. 2007 151:115-27
Species: Human
Sample Type: Cell Lysates
Procalcitonin and proinflammatory cytokine clearance during continuous venovenous haemofiltration in septic patients.
Dahaba AA, Elawady GA, List WF, Rehak PH
Anaesth Intensive Care 2002 30:269-74
Species: Human
Sample Type: Plasma
Rapid secretion of interleukin-1beta by microvesicle shedding.
Dower SK, Kiss-Toth E, MacKenzie A, North RA, Surprenant A, Wilson HL
Immunity 2001 15:825-35
Species: Human
Sample Type: Cell Culture Supernates
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