Porcine IL-1 beta/IL-1F2 Quantikine ELISA Kit | pIL-1b Qkit
掲載日情報：2016/08/18 現在Webページ番号：111376
R&D Systems製のPorcine IL-1 beta/IL-1F2を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年05月01日 10時15分現在]
| 詳細 | 商品名 |
|
文献数 | ||||||||||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
IL-1β, Porcine, ELISA Kit, Quantikine (96well) <IL-1F2, ELISA Kit> |
|
32 | |||||||||||||||||||||||||||||||||||||||||||||||||||
|
|||||||||||||||||||||||||||||||||||||||||||||||||||||
[在庫・価格 ：2024年05月01日 10時15分現在]
IL-1β, Porcine, ELISA Kit, Quantikine (96well) <IL-1F2, ELISA Kit>
文献数： 32
- 商品コード：PLB00B
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
測定範囲：39.10 - 2500 pg/mL，感度：13.6 pg/mL，測定試料：Cell Culture Supernates (25 uL), Serum (50 uL), Heparin Plasma (50 uL)，種交差性：Porcine，検出方法：呈色，測定波長：450 nm，アッセイ法：サンドイッチELISA，アッセイ数：96 well，測定時間：4.5 hours，測定因子：IL-1 beta/IL-1F2 別名：catabolin Genbank No： 3553 |
||
|---|---|---|---|
| 法規制等 | |||
| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
|
||
| 製品記事 |
Helper T Cells Marker Antibodies R&D Systems社 IL-1β関連製品 |
||
| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
||
追加しました。
Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (25 µL), Serum (50 µL), Heparin Plasma (50 µL)
- Sensitivity
- 13.6 pg/mL
- Assay Range
- 39.1 - 2,500 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates)
- Specificity
- Natural and recombinant porcine IL-1 beta
- Interference
- No significant interference observed with available related molecules.
追加しました。
Product Summary
The Quantikine Porcine IL-1 beta Immunoassay is a 4.5 hour solid phase ELISA designed to measure porcine IL-1 beta in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mature porcine IL-1 beta and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant porcine IL-1 beta. Results obtained using natural porcine IL-1 beta show dose response curves that are parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural porcine IL-1 beta.
追加しました。
Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 136 | 348 | 780 | 141 | 338 | 783 |
| Standard Deviation | 9.8 | 17 | 40.9 | 12.2 | 20.5 | 31.7 |
| CV% | 7.2 | 4.9 | 5.2 | 8.7 | 6.1 | 4 |
追加しました。
Recovery
The recovery of porcine IL-1 beta spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 106 | 102-109 |
| Serum (n=4) | 111 | 102-117 |
| Heparin Plasma (n=4) | 111 | 98-117 |
追加しました。
Linearity
To assess the linearity of the assay, samples containing and/or spiked with various concentrations of porcine IL-1 beta in each matrix were diluted with Calibrator Diluent and then assayed.
追加しました。
Additional Infomation
- Molecule Information
- IL-1 beta/IL-1F2
- Aliases
- IL1B; IL-1F2
- Entrez Gene IDs
- 3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 100034237 (Equine); 100135556 (Guinea Pig)
- Background
- IL-1 beta/IL-1F2
- IL-1 is a name that designates two proteins, IL-1 alpha and IL-1 beta, that are the products of distinct genes, but recognize the same cell surface receptors. IL-1 alpha and IL-1 beta are structurally related polypeptides that show approximately 25% homology at the amino acid level. Both proteins are produced by a wide variety of cells in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins.
追加しました。
Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Ornithodoros moubata complement inhibitor is an equally effective C5 inhibitor in pigs and humans.
Barratt-Due A, Lambris JD, Lindstad JK, Lissina O, Mollnes TE, Nunn MA, Pharo A, Thorgersen EB
J. Immunol. 2011 187:4913-9
Species: Porcine
Sample Type: Plasma
CD14 inhibition efficiently attenuates early inflammatory and hemostatic responses in Escherichia coli sepsis in pigs.
Barratt-Due A, Castellheim A, Fosse E, Fure H, Hellerud BC, Johansen HT, Lindstad JK, Mollnes TE, Nielsen EW, Pharo A, Thorgersen EB, Tonnessen TI
FASEB J. 2010 24:712-22
Species: Porcine
Sample Type: Plasma
Plasma concentrations of inflammatory cytokines rise rapidly during ECMO-related SIRS due to the release of preformed stores in the intestine.
Kurundkar AR, McILwain RB, Timpa JG
Lab. Invest. 2010 90:128-39
Species: Porcine
Sample Type: Plasma
Dissecting the effects of lipopolysaccharides from nonlipopolysaccharide molecules in experimental porcine meningococcal sepsis.
Brandtzaeg P, Castellheim A, Hellerud BC, Lindstad JK, Mollnes TE, Nielsen EW, Pharo A, Thorgersen EB, Tonnessen TI
Crit. Care Med. 2010 38:1467-74
Species: Porcine
Sample Type: Plasma
Inhibition of complement and CD14 attenuates the Escherichia coli-induced inflammatory response in porcine whole blood.
Axelsen AK, Gaustad P, Haverson K, Kotwal GJ, Mollnes TE, Pharo A, Sfyroera G, Thorgersen EB
Infect. Immun. 2009 77:725-32
Species: Porcine
Sample Type: Whole Blood
Metabolic activity of the enteric microbiota influences the fatty acid composition of murine and porcine liver and adipose tissues.
Coakley M, Fitzgerald GF, Hart O, Kiely B, Lawlor P, O'Mahony C, O'Mahony L, Quigley EM, Ross RP, Shanahan F, Stanton C, Wall R
Am. J. Clin. Nutr. 2009 89:1393-401
Species: Porcine
Sample Type: Cell Culture Supernates
Cardiac Function and the Proinflammatory Cytokine Response After Recovery From Cardiac Arrest in Swine.
Filler SG, Lewis RJ, Niemann JT, Phan QT, Rosborough JP, Shah AP, Youngquist S
J. Interferon Cytokine Res. 2009 0:
Species: Porcine
Sample Type: Plasma
Cytokine secretion depends on Galalpha(1,3)Gal expression in a pig-to-human whole blood model.
Haraldsen G, Lambris JD, Magotti P, Mollnes TE, Saethre M, Schneider MK, Seebach JD
J. Immunol. 2008 180:6346-53
Species: Porcine
Sample Type: Cell Culture Supernates
Effects of glucocorticoids in ventilated piglets with severe pneumonia.
Absi R, Agusti C, Baquero S, Bassi N, Gando S, Luna CM, Morato JG, Patron JR, Sibila O, Torres A
Eur. Respir. J. 2008 32:1037-46
Species: Porcine
Sample Type: Serum
Blood concentrations of the cytokines IL-1beta, IL-6, IL-10, TNF-alpha and IFN-gamma during experimentally induced swine dysentery.
Essen-Gustavsson B, Fossum C, Jensen-Waern M, Kruse R
Acta Vet. Scand. 2008 50:32
Species: Porcine
Sample Type: Serum
追加しました。
製品情報は掲載時点のものですが、価格表内の価格については随時最新のものに更新されます。お問い合わせいただくタイミングにより製品情報・価格などは変更されている場合があります。
表示価格に、消費税等は含まれていません。一部価格が予告なく変更される場合がありますので、あらかじめご了承下さい。