Mouse sVCAM-1/CD106 Quantikine ELISA Kit | msVCAM-1 Qkit
掲載日情報：2016/08/18 現在Webページ番号：111365
R&D Systems製のMouse sVCAM-1/CD106を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年05月18日 00時00分現在]
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VCAM-1, Soluble, Mouse, ELISA Kit, Quantikine M (96 well) |
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[在庫・価格 ：2024年05月18日 00時00分現在]
VCAM-1, Soluble, Mouse, ELISA Kit, Quantikine M (96 well)
文献数： 27
- 商品コード：MVC00
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：医薬用外毒物
| 説明文 |
測定範囲：0.31 - 20 ng/mL，感度：0.06 ng/mL，測定試料：Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL)，種交差性：Mouse，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，測定時間：4.5 hours，測定因子：VCAM-1/CD106 別名：CD106 Genbank No： 7412 |
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| 法規制等 | 医薬用外毒物 | ||
| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 |
ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL)
- Sensitivity
- 0.06 ng/mL
- Assay Range
- 0.31 - 20 ng/mL (Serum, Cell Culture Supernates, EDTA Plasma)
- Specificity
- Natural and recombinant mouse sVCAM-1
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Mouse soluble VCAM-1 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse sVCAM-1 levels in cell culture supernates, serum, and EDTA plasma. It contains NS0-expressed recombinant mouse sVCAM-1 and antibodies raised against the recombinant protein. Results obtained for naturally occurring mouse sVCAM-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural mouse sVCAM-1.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 0.96 | 2.84 | 8.55 | 0.87 | 2.64 | 8.5 |
| Standard Deviation | 0.05 | 0.13 | 0.37 | 0.07 | 0.18 | 0.63 |
| CV% | 5.2 | 4.6 | 4.3 | 8 | 6.8 | 7.4 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 0.93 | 2.61 | 8.58 | 0.86 | 2.46 | 8.46 |
| Standard Deviation | 0.07 | 0.17 | 0.4 | 0.05 | 0.11 | 0.65 |
| CV% | 7.5 | 6.5 | 4.7 | 5.8 | 4.5 | 7.7 |
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Recovery
The recovery of mouse sVCAM-1 spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=10) | 99 | 80-118 |
| Cell Culture Supernates (n=6) | 100 | 89-112 |
| EDTA Plasma (n=4) | 100 | 81-119 |
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Linearity
To assess the linearity of the assay, six samples of each matrix containing and/or spiked with high concentrations of mouse sVCAM-1 were diluted with Calibrator Diluent and then assayed.
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Additional Infomation
- Molecule Information
- VCAM-1/CD106
- Aliases
- CD106
- Entrez Gene IDs
- 7412 (Human); 22329 (Mouse)
- Background
- VCAM-1
- Human VCAM-1 (CD106), a member of the immunoglobulin superfamily, is a cell surface protein expressed by activated endothelial cells and certain leukocytes such as macrophages. VCAM-1 expression is induced by IL-1beta, IL-4, TNF-alpha and IFN-gamma. VCAM-1 binds to leukocyte integrins VLA-4 and Integrin alpha 4 beta 7.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Early protection from burn-induced acute lung injury by deletion of preprotachykinin-A gene.
Lu J, Moochhala S, Sio SW
Am. J. Respir. Crit. Care Med. 2010 181:36-46
Species: Mouse
Sample Type: Tissue Homogenates
Early atherosclerosis and vascular inflammation in mice with diet-induced type 2 diabetes.
Bang CA, Bartels ED, Nielsen LB
Eur. J. Clin. Invest. 2009 39:190-9
Species: Mouse
Sample Type: Plasma
Alternative implication of CXCR4 in JAK2/STAT3 activation in small cell lung cancer.
Burger M, Catusse J, Hartmann TN, Leick M, Pfeiffer M, Schmitt-Graeff A
Br. J. Cancer 2009 100:1949-56
Species:
Sample Type: Cell Culture Supernates
Vascular and inflammatory stresses mediate atherosclerosis via RAGE and its ligands in apoE-/- mice.
Belov D, Bu DX, Chang JS, Hallam K, Harja E, Hudson BI, Kalea AZ, Lalla E, Lu Y, Nikolla Z, Oruganti S, Ramasamy R, Rosario RH, Schmidt AM, Shen X, Yan SF
J. Clin. Invest. 2008 118:183-94
Species: Mouse
Sample Type: Plasma
Vascular dysfunction in a murine model of severe hemolysis.
Fagan KA, Frei AC, Guo Y, Hogg N, Jones DW, Pritchard KA, Wandersee NJ
Blood 2008 112:398-405
Species: Mouse
Sample Type: Plasma
Aspergillus fumigatus stimulates leukocyte adhesion molecules and cytokine production by endothelial cells in vitro and during invasive pulmonary disease.
Chiang LY, Filler SG, Gravelat FN, Patterson TF, Sheppard DC
Infect. Immun. 2008 76:3429-38
Species: Mouse
Sample Type: Tissue Homogenates
Prevention of murine antiphospholipid syndrome by BAFF blockade.
Bethunaickan R, Davidson A, Factor SM, Huang W, Kahn P, Madaio MP, Ramanujam M, Tao H
Arthritis Rheum. 2008 58:2824-34
Species: Mouse
Sample Type: Serum
A neutralizing antibody against receptor for advanced glycation end products (RAGE) reduces atherosclerosis in uremic mice.
Bang CA, Binder CJ, Bro S, Denner L, Flyvbjerg A, Nielsen LB, Olgaard K
Atherosclerosis 2008 201:274-80
Species: Mouse
Sample Type: Plasma
Cell-based angiopoietin-1 gene therapy for acute lung injury.
Deng Y, Dumont DJ, Han RN, Hood RD, Lai PF, McCarter SD, Mei SH, Parker CH, Stewart DJ, Suen RS, Zhang QW, Zhao YD
Am. J. Respir. Crit. Care Med. 2007 175:1014-26
Species: Mouse
Sample Type: Plasma
Atheroprotective role of interleukin-6 in diet- and/or pathogen-associated atherosclerosis using an ApoE heterozygote murine model.
Amar S, Bishayi B, Hoge M, Madan M
Atherosclerosis 2007 197:504-14
Species: Mouse
Sample Type: Serum
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