Mouse Dkk-1 Quantikine ELISA Kit | mDkk-1 Qkit
掲載日情報：2016/08/18 現在Webページ番号：111312
R&D Systems製のMouse Dkk-1を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年05月18日 00時00分現在]
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Dkk-1, Mouse, ELISA Kit, Quantikine (96 well) |
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[在庫・価格 ：2024年05月18日 00時00分現在]
Dkk-1, Mouse, ELISA Kit, Quantikine (96 well)
文献数： 5
- 商品コード：MKK100
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
測定範囲：62.50 - 4000 pg/mL，感度：29.6 pg/mL，測定試料：Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)，種交差性：Mouse，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，測定時間：4.5 hours，測定因子：Dkk-1 別名：dickkopf (Xenopus laevis) homolog 1 Genbank No： 22943 |
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| 保存条件 | 4℃ | 法規備考 | |
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| 製品記事 |
ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)
- Sensitivity
- 29.6 pg/mL
- Assay Range
- 62.5 - 4,000 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma)
- Specificity
- Natural and recombinant mouse Dkk-1
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Mouse Dkk-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure Dkk-1 in cell culture supernates, mouse serum, and plasma. It contains NS0-expressed recombinant mouse Dkk-1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse Dkk-1. Results obtained using natural mouse Dkk-1 showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse Dkk-1.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 230 | 478 | 1,487 | 219 | 481 | 1,492 |
| Standard Deviation | 20.1 | 29.7 | 80.8 | 20.8 | 44.7 | 144 |
| CV% | 8.7 | 6.2 | 5.4 | 9.5 | 9.3 | 9.7 |
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Recovery
The recovery of mouse Dkk-1 spiked to three levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=4) | 113 | 100-120 |
| Heparin Plasma (n=4) | 113 | 104-119 |
| Cell Culture Supernates (n=4) | 99 | 88-107 |
| EDTA Plasma (n=4) | 109 | 93-117 |
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Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of mouse Dkk-1 in each matrix were diluted with Calibrator Diluent and assayed.
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Additional Infomation
- Molecule Information
- Dkk-1
- Aliases
- undefined
- Entrez Gene IDs
- 22943 (Human); 13380 (Mouse)
- Background
- Dkk Family
- Members of the dickkopf-related protein family (Dkk-1, -2, -3, and -4) are secreted proteins with two cysteine-rich domains separated by a linker region. Dkk-3 and -4 also have one prokineticin domain. Dkk-1, -2, and -4 function as antagonists of canonical Wnt signaling by binding to LRP5/6, preventing interaction of LRP5/6 with Wnt-Frizzled complexes. Dkk-1, -2, and -4 also bind cell surface Kremen-1 or -2 and promote the internalization of LRP5/6. Antagonistic activity of Dkk-3 has not been demonstrated. Dkk proteins have distinct patterns of expression in adult and embryonic tissues and have a wide range of effects on tissue development and morphogenesis. The Dkk family also includes Soggy, which is homologous to Dkk-3 but not to the other family members. Soggy has not been shown to inhibit Wnt signaling, and its role in the pathway is unclear.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Bone marrow stromal cells create a permissive microenvironment for myeloma development: a new stromal role for Wnt inhibitor Dkk1.
Edwards C, Fowler J, Lwin S, Mundy G
Cancer Res 2012 72:2183-9
Species: Mouse
Sample Type: Serum
Prostaglandin E(2) Signals Through PTGER2 to Regulate Sclerostin Expression.
Genetos DC, Loots GG, Yellowley CE
PLoS ONE 2011 6:e17772
Species: Mouse
Sample Type: Cell Lysates
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