Human TIMP-4 Quantikine ELISA Kit | hTIMP-4 QKit
掲載日情報：2016/08/18 現在Webページ番号：111213
R&D Systems製のHuman TIMP-4を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年05月18日 00時00分現在]
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TIMP-4, Human, ELISA Kit, Quantikine (96well) |
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[在庫・価格 ：2024年05月18日 00時00分現在]
TIMP-4, Human, ELISA Kit, Quantikine (96well)
文献数： 5
- 商品コード：DTM400
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：カルタヘナ該当品
| 説明文 |
測定範囲：78.10 - 5000 pg/mL，感度：10 pg/mL，測定試料：Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Human Milk (10 uL)，種交差性：Human，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC24，測定時間：4.5 hours，測定因子：TIMP-4 別名：metalloproteinase inhibitor 4 Genbank No： 7079 |
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| 法規制等 | カルタヘナ該当品 | ||
| 保存条件 | 4℃ | 法規備考 | |
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| 製品記事 |
ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- undefined
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL), Human Milk (10 µL)
- Sensitivity
- 10 pg/mL
- Assay Range
- 78.1 - 5,000 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, Human Milk, EDTA Plasma)
- Specificity
- Natural and recombinant human TIMP-4. This kit detects recombinant human TIMP-4 in both free and MMP-bound forms.
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human TIMP-4 Immunoassay is a 4.5 hour solid phase ELISA designed to measure TIMP-4 levels in cell culture supernates, serum, plasma, and human milk. It contains Sf 21-expressed recombinant human TIMP-4 and antibodies raised against the recombinant protein. This kit detects recombinant human TIMP-4 in both free and MMP-bound forms. Natural human TIMP-4 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural human TIMP-4.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Heparin Plasma, Human Milk
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 235 | 768 | 1,516 | 220 | 712 | 1,414 |
| Standard Deviation | 13.2 | 21.5 | 70.4 | 20.2 | 48.2 | 87.6 |
| CV% | 5.6 | 2.8 | 4.6 | 9.2 | 6.8 | 6.2 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 252 | 793 | 1,476 | 232 | 708 | 1,377 |
| Standard Deviation | 11 | 46.9 | 77.8 | 20.8 | 45.8 | 96.4 |
| CV% | 4.4 | 5.9 | 5.3 | 9 | 6.5 | 7 |
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Recovery
The recovery of TIMP-4 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=4) | 96 | 86-103 |
| Heparin Plasma (n=4) | 93 | 86-104 |
| Cell Culture Supernates (n=4) | 96 | 87-105 |
| EDTA Plasma (n=4) | 95 | 87-113 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of TIMP-4 were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- TIMP-4
- Aliases
- undefined
- Entrez Gene IDs
- 7079 (Human); 110595 (Mouse)
- Background
- TIMPs
- TIMPs-1 through -4 regulate the activity of zinc metalloproteases known as MMPs, ADAMs andADAMTSs.Structurally, TIMPs contain two domains. The N-terminal domain binds to the activesite of mature metalloproteases via a 1:1 non-covalent interaction, blocking access of substrates to thecatalytic site.In addition, The C-terminal domain of TIMP-1 and TIMP-2 binds to the hemopexin- like domain of pro-MMP-9 and pro-MMP-2, respectively.The latter binding is essential for the cellsurface activation of MMP-2 by MMP-14.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Circulating biomarkers of tissue remodelling in pulmonary hypertension.
Frank H, Krahn T, Kramer F, Kropf C, Kruska L, Lepper PM, Rudiger S, Schneiderbauer R, Schumann C, Stoiber KM, Wibmer T
Biomarkers 2010 15:523
Species: Human
Sample Type: Plasma
Primary human acute myelogenous leukemia cells release matrix metalloproteases and their inhibitors: release profile and pharmacological modulation.
Bruserud O, Hatfield KJ, Kalland KH, Kittang AO, Oyan AM, Reikvam H
Eur. J. Haematol. 2010 84:239-51
Species: Human
Sample Type: Cell Culture Supernates
Profile of macrophages in human abdominal aortic aneurysms: a transcriptomic, proteomic, and antibody protein array study.
Amouyel P, Beseme O, Chwastyniak M, Drobecq H, Dubois E, Hot D, Koussa M, Lamblin N, Lemoine Y, Pinet F, Ratajczak P
J. Proteome Res. 2010 9:3720-9
Species: Human
Sample Type: Plasma
Imbalance of tissue inhibitors of metalloproteinases (TIMP) - 1 and - 4 serum levels, in patients with inflammatory bowel disease.
Davidi IP, Germenis AE, Kapsoritaki AI, Kapsoritakis AN, Lotis VD, Manolakis AC, Mylonis PI, Potamianos SP, Theodoridou AT
BMC Gastroenterol 2008 8:55
Species: Human
Sample Type: Serum
Specific temporal profile of matrix metalloproteinase release occurs in patients after myocardial infarction: relation to left ventricular remodeling.
Ahmed SH, Bonnema DD, Clark LL, Corn WC, Finklea L, Leonardi AH, McClure CD, Spinale FG, Stroud RE, Webb CS, Zile MR
Circulation 2006 114:1020-7
Species: Human
Sample Type: Plasma
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