Human sTfR Quantikine IVD ELISA Kit | "hsTfR QKit, IVD"
掲載日情報：2016/08/18 現在Webページ番号：111207
R&D Systems製のHuman sTfR Quantikine IVD ELISA Kitを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月27日 00時00分現在]
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Transferrin Receptor, Soluble, Human, ELISA Kit, Quantikine (96well) <sTfR, Human, ELISA Kit> |
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[在庫・価格 ：2025年04月27日 00時00分現在]
Transferrin Receptor, Soluble, Human, ELISA Kit, Quantikine (96well) <sTfR, Human, ELISA Kit>
文献数： 42
- 商品コード：DTFR1
- メーカー：RSD
- 包装：1kit
- 本製品は取扱中止になりました
| 説明文 | Genbank No：
7037
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| 法規制等 | |||
| 保存条件 | 法規備考 | ||
| 掲載カタログ |
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| 製品記事 | Human Soluble Transferrin Receptor ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 2.5 hours
- Sample Type & Volume Required Per Well
- Serum (20 µL), EDTA Plasma (20 µL), Heparin Plasma (20 µL), Citrate Plasma (20 µL)
- Sensitivity
- 0.5 nmol/L
- Assay Range
- 3 - 80 nmol/L (Serum, Heparin Plasma, Citrate Plasma, EDTA Plasma)
- Specificity
- Measures human sTfR levels in various samples
- Interference
- Interference observed with other substances.
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Product Summary
The Quantikine IVD Human sTfR Immunoassay is designed for the quantitative determination of soluble transferrin receptor (sTfR) concentrations in human serum and plasma as an aid in the diagnosis of Iron-Deficiency Anemia (IDA), especially for the differential diagnosis of IDA and Anemia of Chronic Disease (ACD).
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 60 | 60 | 60 |
| Mean | 6.9 | 18.2 | 40.2 | 10.9 | 25.7 | 67.2 |
| Standard Deviation | 0.3 | 1.3 | 2.5 | 0.7 | 1.4 | 3.8 |
| CV% | 4.3 | 7.1 | 6.2 | 6.4 | 5.4 | 5.7 |
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Recovery
The recovery of sTfR spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=5) | 97 | 95-100 |
| Heparin Plasma (n=5) | 106 | 101-112 |
| Citrate Plasma (n=5) | 112 | 105-121 |
| EDTA Plasma (n=5) | 103 | 97-112 |
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Linearity
To assess the linearity of the assay, five samples containing high concentrations of sTfR were diluted with Specimen Diluent and evaluated._DTFR1_ELISA_191.jpg){kind=spacer}
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Additional Infomation
- Molecule Information
- TfR (Transferrin R)
- Aliases
- CD71; TFR1; TFRC; TRFR
- Entrez Gene IDs
- 7037 (Human); 22042 (Mouse); 64678 (Rat)
- Background
- Transferrin Receptors
- Transferrin receptors are transmembrane, homodimeric proteins that play a role in iron transport, uptake, and storage. TfR is an essential receptor, found on almost all cell types, regulated by intracellular iron concentration, and has a high affiinity for transferrin. Soluble TfR (sTfR) arises from the proteolysis of TfR into monomers that can be measured in plasma and serum. TfR2 has limited tissue distribution (hepatocytes and enterocytes), is regulated by the cell cycle, and binds transferrin poorly.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Regulation of hepcidin expression at high altitude.
Dorrington KL, Lakhal S, Leon-Velarde F, Mole DR, Nickol AH, Privat C, Rivera-Ch M, Robbins PA, Smith TG, Talbot NP
Blood 2012 119:857-60
Species: Human
Sample Type: Plasma
Comparison between one and three doses a week of recombinant erythropoietin in very low birth weight infants.
Bonillo A, Diez J, Galera R, Gonzalez-Ripoll M, Lendinez F, Llamas MA, Sanchez AR, Vazquez Lopez MA
J Perinatol 2011 31:118-24
Species: Human
Sample Type: Serum
Modulation of hepcidin production during hypoxia-induced erythropoiesis in humans in vivo: data from the HIGHCARE project.
Galimberti S, Mariani R, Piperno A
Blood 2011 117:2953-9
Species: Human
Sample Type: Serum
Randomized, double-blind, placebo-controlled trial of an iron-fortified food product in female soldiers during military training: relations between iron status, serum hepcidin, and inflammation.
Cable SJ, Karl JP, Lieberman HR, McClung JP, Williams KW, Young AJ
Am. J. Clin. Nutr. 2010 92:93-100
Species: Human
Sample Type: Serum
Body iron excretion by healthy men and women.
Hunt JR, Johnson LK, Zito CA
Am. J. Clin. Nutr. 2009 89:1792-8
Species: Human
Sample Type: Serum
Time course of anemia during six months follow up following intensive care discharge and factors associated with impaired recovery of erythropoiesis.
Bateman AP, McArdle F, Walsh TS
Crit. Care Med. 2009 37:1906-12
Species: Human
Sample Type: Serum
Randomized, double-blind, placebo-controlled trial of iron supplementation in female soldiers during military training: effects on iron status, physical performance, and mood.
Cable SJ, Karl JP, Lieberman HR, McClung JP, Nindl BC, Williams KW, Young AJ
Am. J. Clin. Nutr. 2009 90:124-31
Species: Human
Sample Type: Serum
Mortality in HIV infection is independently predicted by host iron status and SLC11A1 and HP genotypes, with new evidence of a gene-nutrient interaction.
Bates C, Hennig BJ, Hill AV, Jaye A, McDermid JM, Prentice AM, Sirugo G, Todd J, van der Loeff MF, Whittle HC
Am. J. Clin. Nutr. 2009 90:225-33
Species: Human
Sample Type: Plasma
Retinol and riboflavin supplementation decreases the prevalence of anemia in chinese pregnant women taking iron and folic Acid supplements.
Han XX, Jiang DC, Kok FJ, Ma AG, Schouten EG, Sun YY, Yang F, Zhang FZ
J. Nutr. 2008 138:1946-50
Species: Human
Sample Type: Plasma
Tumor necrosis factor SNP haplotypes are associated with iron deficiency anemia in West African children.
Atkinson SH, Bejon PA, Hanchard N, Kwiatkowski DP, Morgan G, O'Connell MA, Prentice AM, Rockett KA, Sirugo G
Blood 2008 112:4276-83
Species: Human
Sample Type: Plasma
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