Human CD117/c-kit Quantikine ELISA Kit | hCD117/c-kit QKit
掲載日情報:2016/08/18 現在Webページ番号:111192
R&D Systems製のHuman CD117/c-kitを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 :2025年04月27日 00時00分現在]
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SCF Receptor, Soluble, Human, ELISA Kit, Quantikine (96 well) <c-kit, ELISA Kit> |
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[在庫・価格 :2025年04月27日 00時00分現在]
SCF Receptor, Soluble, Human, ELISA Kit, Quantikine (96 well) <c-kit, ELISA Kit>
文献数: 6
- 商品コード:DSCR00
- メーカー:RSD
- 包装:1kit
- 本製品は取扱中止になりました
説明文 | Genbank No:
3815
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法規制等 | カルタヘナ該当品 | ||
保存条件 | 法規備考 | ||
掲載カタログ |
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製品記事 | がん幹細胞関連因子測定用 Quantikine ELISA Kit ELISA Kit製品に関するFAQ(R&D Systems) |
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関連記事 | R&D Systems Quantikine ELISA Kit/DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL)
- Sensitivity
- 0.339 ng/mL
- Assay Range
- 1.56 - 50 ng/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma)
- Specificity
- Natural and recombinant human SCF sR
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human SCF sR Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human SCF sR in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant human SCF sR and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human SCF sR showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human SCF sR.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 40 | 40 | 40 |
Mean | 4.69 | 14.1 | 25.7 | 5.9 | 15.1 | 28.7 |
Standard Deviation | 0.298 | 0.359 | 0.562 | 0.555 | 0.688 | 1.76 |
CV% | 6.4 | 2.5 | 2.2 | 9.4 | 4.6 | 6.1 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 40 | 40 | 40 |
Mean | 5.68 | 13.8 | 26.4 | 5.32 | 13.6 | 26.9 |
Standard Deviation | 0.228 | 0.39 | 0.707 | 0.481 | 0.509 | 1.21 |
CV% | 4 | 2.8 | 2.7 | 9 | 3.7 | 4.5 |
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Recovery
The recovery of SCF sR spiked to levels throughout the range of the assay in various matrices was evaluated.
Sample Type | Average % Recovery | Range % |
Serum (n=4) | 93 | 86-115 |
Heparin Plasma (n=4) | 92 | 87-102 |
EDTA Plasma (n=4) | 91 | 84-106 |
Cell Culture Media (n=4) | 103 | 97-110 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of SCF sR were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- CD117/c-kit
- Aliases
- CD117; c-kit; KIT; PBT; SCF R
- Entrez Gene IDs
- 3815 (Human); 16590 (Mouse); 64030 (Rat)
- Background
- CD117/c-kit (SCF Receptor)
- CD117, the gene product of the c-kit proto-oncogene and its ligand, stem cell factor, play essential roles in gametogenesis, melanogenesis and hematopoiesis. Stem cell factor receptor is a member of the type III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, Flt-3 and PDGF. It is expressed in hematopoietic progenitor cells, normal B and T cell progenitor cells, mast cells, germ cells, melanocytes, neurons, glial cells, placenta, kidney, lung and gut.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Plasma biomarkers as predictors of outcome in patients with advanced hepatocellular carcinoma.
Amadori D, Arvanitakis C, Badulescu F, Barrios C, Beaugrand M, Befeler A, Bennouna J, Blanc J-, Blum H, Bolondi L, Borbath I, Boyer M, Boyer T, Britten C, Bronowicki J-, Bruix J, Buggisch P, Burak K, Bustamante Schneider J, Byrne T, Caspary W, Cisnero Garza LE, Cognetti F, Cosme de Oliveira A, Craxi A, De Greve J, Degos F, Diago M, Dollinger M, Dominguez S, Falk S, Farinati F, Figer A, Forner A, Gamargo Garate C, Gane E, Garcia-Tsao G, Gerken G, Germanidis G, Goke B, Gold P, Goldenberg A, Grange J-, Gregor M, Greten T, Gridelli C, Haussinger D, Heuman D, Hilgard P, Hillon P, Jassem J, Jeffry Evans TR, Katsos I, Kennedy P, Koch A, Kotzev I, Kubyshkin V, Lathia CD, Leon J, Llovet JM, Lozano A, Ma M, Marrero J, Martinez-Castillo J, Martoni A, Mazzaferro V, Meinhardt G, Metrakos P, Meyer T, Miron L, Montero Alvarez JL, Muellhaupt B, O'Donnell A, Olweny C, Pallota MG, Pascual S, Pena CE, Porta C, Raoul J-, Reeves H, Ricci S, Riordan S, Ross P, Roth A, Ru??z del Arbol L, Rydzewska G, Sangiovanni A, Sangro B, Santoro A, Scher??bl J, Scheulen M, Schilsky M, Schmid R, Schwartz J, Schwartz M, Seitz J-, Shan M, Sherman M, Sol?? R, Spengler U, Stemmer S, Strickland A, Szawlowski A, Tabernero J, Takov D, Tchernev K, Tebbutt N, Thomson B, Tomczak P, Trevisani F, Van Laethem J-, Van Steenbergen W, Van Vlierberghe H, Wiest R, Zarba JJ, Zeuzem S
Clin. Cancer Res. 2012 18:2290-300
Species: Human
Sample Type: Plasma
Efficacy, safety, and potential biomarkers of sunitinib monotherapy in advanced hepatocellular carcinoma: a phase II study.
Abrams TA, Ancukiewicz M, Bhargava P, Blaszkowsky LS, Catalano OA, Clark JW, di Tomaso E, Duda DG, Enzinger PC, Fuchs CS, Hezel AF, Jain RK, Kwak EL, Lahdenranta J, Meyerhardt J, Miksad R, Ryan DP, Sahani DV, Sindhwani V, Yoon SS, Zhu AX
J. Clin. Oncol. 2009 27:3027-35
Species: Human
Sample Type: Plasma
Endothelial progenitor cell levels in obese men with the metabolic syndrome and the effect of simvastatin monotherapy vs. simvastatin/ezetimibe combination therapy.
de Bree P, Doevendans PA, Hajer GR, Hoefer IE, Olijhoek JK, Rafii S, Verhaar MC, Visseren FL, Westerweel PE
Eur. Heart J. 2008 29:2808-17
Species: Human
Sample Type: Serum
Phase II trial of sunitinib in patients with metastatic colorectal cancer after failure of standard therapy.
Belt RJ, Bergsland EK, Chao RC, Chow-Maneval E, DePrimo SE, Eckhardt SG, Haller DG, Huang X, Hurwitz HI, Lenz HJ, Lockhart AC, Marshall JL, Rocha Lima CM, Rosen LS, Saltz LB
J. Clin. Oncol. 2007 25:4793-9
Species: Human
Sample Type: Plasma
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