Human Osteopontin (OPN) Quantikine ELISA Kit | hOPN QKit
掲載日情報：2016/08/18 現在Webページ番号：111164
R&D Systems製のHuman Osteopontin (OPN)を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年05月18日 00時00分現在]
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Osteopontin, Human, ELISA Kit, Quantikine (96 well) <OPN, ELISA Kit> |
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[在庫・価格 ：2024年05月18日 00時00分現在]
Osteopontin, Human, ELISA Kit, Quantikine (96 well) <OPN, ELISA Kit>
文献数： 66
- 商品コード：DOST00
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：2個
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
測定範囲：0.31 - 20 ng/mL，感度：0.024 ng/mL，測定試料：Cell Culture Supernates (50 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (50 uL), Human Milk (50 uL)，種交差性：Human，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC136，測定時間：4.5 hours，測定因子：Osteopontin/OPN 別名：BNSP Genbank No： 6696 |
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| 保存条件 | 4℃ | 法規備考 | |||||
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| 製品記事 |
ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Urine (50 µL), Human Milk (50 µL)
- Sensitivity
- 0.024 ng/mL
- Assay Range
- 0.312 - 20 ng/mL (Heparin Plasma, Cell Culture Supernates, Human Milk, EDTA Plasma, Urine)
- Specificity
- Natural and recombinant human Osteopontin
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human Osteopontin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Osteopontin in cell culture supernates, plasma, urine, and human milk. It contains NS0-expressed recombinant human Osteopontin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Osteopontin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Osteopontin.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Urine, Human Milk
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 2.3 | 4.9 | 9.3 | 2.36 | 4.81 | 9.17 |
| Standard Deviation | 0.092 | 0.126 | 0.267 | 0.155 | 0.274 | 0.499 |
| CV% | 4 | 2.6 | 2.9 | 6.6 | 5.7 | 5.4 |
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Recovery
The recovery of Osteopontin spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Heparin Plasma (n=4) | 101 | 95-112 |
| EDTA Plasma (n=4) | 102 | 92-114 |
| Cell Culture Media (n=4) | 104 | 86-113 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Osteopontin were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- Osteopontin/OPN
- Aliases
- Eta-1; OPN; Spp1
- Entrez Gene IDs
- 6696 (Human); 20750 (Mouse); 25353 (Rat); 281499 (Bovine)
- Background
- Osteopontin (OPN)
- Osteopontin (OPN), previously referred to as transformation-associated secreted phosphoprotein, bone sialoprotein I, and minopotin, is a secreted, highly acidic, calcium-binding, RGD-containing, phosphorylated glycoprotein originally isolated from bone matrix. Subsequently, OPN has been found in kidney, placenta, blood vessels, and various tumor tissues. Human, mouse, rat, porcine, and bovine OPN share approximately 40% amino acid sequence identity.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Osteopontin is a prognostic biomarker in non-small cell lung cancer.
Berge G, Boye K, Brustugun O, Halvorsen A, Helland A, Lund-Iversen M, Maelandsmo G, Oijordsbakken M, Rud A, Solberg S
BMC Cancer 2013 13:540
Species: Human
Sample Type: Serum
Osteopontin is a prognostic factor for survival of acute myeloid leukemia patients.
Appelmann I, Bayer M, Berdel WE, Biermann C, Buchner T, Gerss J, Hiddemann W, Kessler T, Liersch R, Lowenberg B, Mesters R, Muller-Tidow C, Schliemann C, Schwoppe C
Blood 2012 119:5215-20
Species: Human
Sample Type: Plasma
A multi-analyte assay for the non-invasive detection of bladder cancer.
Chang , Myron, Dai , Yunfeng, Goodison , Steve, Rosser , Charles , Urquidi , Virginia
PLoS ONE 2012 7:e47469
Species: Human
Sample Type: Urine
Prognostic utility of pre-operative circulating osteopontin, carbonic anhydrase IX and CRP in renal cell carcinoma.
Br. J. Cancer 2012 107:1131-7
Plasma markers for identifying patients with metastatic melanoma.
Ariyan S, Bacchiocchi A, Halaban R, Hoyt K, Kirsch J, Kluger HM, Kluger Y, Mayer T, Molinaro A, Sznol M
Clin. Cancer Res. 2011 17:2417-25
Species: Human
Sample Type: Plasma
An in vivo platform for tumor biomarker assessment.
Colovos C, Servais EL, Suzuki K
PLoS ONE 2011 6:e26722
Species: Human
Sample Type: Plasma
Serum levels of beta-catenin as a potential marker for genotype 4/hepatitis C-associated hepatocellular carcinoma.
Alam El-Din H, Bahnassy A, Daoud S, Moharram N, Morsy H, Shaarawy S, Zekri A
Oncol Rep 2011 26:825-31
Osteopontin as a novel prognostic marker in stable ischaemic heart disease: a 3-year follow-up study.
Boudoulas H, Capetanaki Y, Georgiadou P, Gizas V, Iliodromitis EK, Kolokathis F, Kremastinos DT, Mavroidis M, Varounis C
Eur. J. Clin. Invest. 2010 0:
Species: Human
Sample Type: Plasma
Circulating levels of osteopontin are closely related to glomerular filtration rate and cardiovascular risk markers in patients with chronic kidney disease.
Bode-Boeger SM, Fliser D, Haller H, Kielstein JT, Kliem V, Kramer R, Lorenzen J, Veldink H
Eur. J. Clin. Invest. 2010 40:294-300
Species: Human
Sample Type: Plasma
Osteopontin is expressed and functional in human eosinophils.
Berkman N, Puxeddu I, Ribatti D
Allergy 2010 65:168-74
Species: Human
Sample Type: BALF
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