Human Total MMP-3 Quantikine ELISA Kit | hTot MMP-3 Qkit
掲載日情報：2016/08/18 現在Webページ番号：111152
R&D Systems製のHuman Total MMP-3を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年05月22日 11時55分現在]
| 詳細 | 商品名 |
|
文献数 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
MMP-3, Total, Human, ELISA Kit, Quantikine (96 well) |
|
55 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
[在庫・価格 ：2024年05月22日 11時55分現在]
MMP-3, Total, Human, ELISA Kit, Quantikine (96 well)
文献数： 55
- 商品コード：DMP300
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
測定範囲：0.16 - 10 ng/mL，感度：0.045 ng/mL，測定試料：Cell Culture Supernates (50 uL), Serum (10 uL), Heparin Plasma (10 uL)，種交差性：Human，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC127，測定時間：4.5 hours，測定因子：MMP-3 別名：CHDS6 Genbank No： 4314 |
||||||
|---|---|---|---|---|---|---|---|
| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | 4℃ | 法規備考 | |||||
| 掲載カタログ |
|
||||||
| 製品記事 |
ELISA Kit製品に関するFAQ（R&D Systems） |
||||||
| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
||||||
追加しました。
Product Details
- Assay Type
- undefined
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (10 µL), Heparin Plasma (10 µL)
- Sensitivity
- 0.045 ng/mL
- Assay Range
- 0.156 - 10 ng/mL (Serum, Heparin Plasma, Cell Culture Supernates)
- Specificity
- Natural and recombinant human total MMP-3
- Interference
- No significant interference observed with available related molecules.
追加しました。
Product Summary
The Quantikine Human Total MMP-3 Immunoassay is a 4.5 hour solid phase immunoassay designed to measure total MMP-3 (pro- and active MMP-3) in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human Pro-MMP-3 and antibodies raised against the recombinant factor. Both antibodies also recognize recombinant human active MMP-3. Natural human MMP-3 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural human MMP-3.
追加しました。
Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 0.962 | 3.21 | 5.87 | 0.994 | 3.08 | 5.7 |
| Standard Deviation | 0.062 | 0.197 | 0.332 | 0.085 | 0.217 | 0.449 |
| CV% | 6.4 | 6.1 | 5.7 | 8.6 | 7 | 7.9 |
追加しました。
Recovery
The recovery of MMP-3 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=5) | 90 | 86-96 |
| Heparin Plasma (n=5) | 92 | 85-106 |
| Cell Culture Media (n=4) | 96 | 87-104 |
追加しました。
Linearity
To assess the linearity of the assay, samples containing high concentrations of MMP-3 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
追加しました。
Additional Infomation
- Molecule Information
- MMP-3
- Aliases
- Stromelysin 1
- Entrez Gene IDs
- 4314 (Human); 17392 (Mouse)
- Background
- Matrix Metalloproteinases (MMPs)
- The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).
追加しました。
Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Validation and quality control of ELISAs for the use with human saliva samples.
Jaedicke KM, Preshaw PM, Taylor JJ
J. Immunol. Methods 2012 377:62-5
Interleukin-6 and matrix metalloproteinase expression in the subretinal fluid during proliferative vitreoretinopathy: correlation with extent, duration of RRD and PVR grade.
Androudi S, Brazitikos P, Dimitrakos SA, Diza E, Karakiulakis G, Papakonstantinou E, Symeonidis C, Tsaousis KT, Tsinopoulos I
Cytokine 2012 59:184-90
Cyclooxygenase-2 inhibitor blocks the production of West Nile virus-induced neuroinflammatory markers in astrocytes.
Kumar M, Nerurkar VR, Verma S
J. Gen. Virol. 2011 92:507-15
Species: Human
Sample Type: Cell Culture Supernates
Interleukin-6 and the matrix metalloproteinase response in the vitreous during proliferative vitreoretinopathy.
Androudi S, Brazitikos P, Dimitrakos SA, Diza E, Karakiulakis G, Papakonstantinou E, Rotsos T, Symeonidis C
Cytokine 2011 54:212-7
Species: Human
Sample Type: Vitreous Humor
Proteolytic release of the receptor for advanced glycation end products from in vitro and in situ alveolar epithelial cells.
Makita K, Matthay MA, Uchida T, Yamakawa N
Am. J. Physiol. Lung Cell Mol. Physiol. 2011 300:L516-25
Species: Human
Sample Type: pulmonary edema fluid
Primary human acute myelogenous leukemia cells release matrix metalloproteases and their inhibitors: release profile and pharmacological modulation.
Bruserud O, Hatfield KJ, Kalland KH, Kittang AO, Oyan AM, Reikvam H
Eur. J. Haematol. 2010 84:239-51
Species: Human
Sample Type: Cell Culture Supernates
Elevated serum matrix metalloproteinase-3 and -7 in H. pylori-related gastric cancer can be biomarkers correlating with a poor survival.
Cheng HC, Sheu BS, Wang YL, Wu JJ, Yang HB, Yeh YC
Dig. Dis. Sci. 2010 55:1649-57
Species: Human
Sample Type: Serum
Circulating matrix metalloproteinase-3 and metalloproteinase-9 and tissue Doppler measures of diastolic dysfunction to risk stratify patients with systolic heart failure.
Ballo P, Buralli S, Conti U, Dini FL, Duranti E, Fontanive P, Marzilli M, Metelli MR, Taddei S
Am. J. Cardiol. 2010 105:853-6
Species: Human
Sample Type: Plasma
Soluble biomarkers of cartilage and bone metabolism in early proof of concept trials in psoriatic arthritis: effects of adalimumab versus placebo.
DeGroot J, Koeman RC, van Kuijk AW
PLoS ONE 2010 5:e12556
Species: Human
Sample Type: Serum
Matrix metalloproteinase-9 and plasminogen activator inhibitor-1 are associated with right ventricular structure and function: the MESA-RV Study.
Barr RG, Johnson WC, Kawut SM
Biomarkers 2010 15:731-8
Species: Human
Sample Type: Plasma
追加しました。
製品情報は掲載時点のものですが、価格表内の価格については随時最新のものに更新されます。お問い合わせいただくタイミングにより製品情報・価格などは変更されている場合があります。
表示価格に、消費税等は含まれていません。一部価格が予告なく変更される場合がありますので、あらかじめご了承下さい。