Human HTRA2/Omi Quantikine ELISA Kit | hHTRA2/Omi QKit
掲載日情報：2016/08/18 現在Webページ番号：111117
R&D Systems製のHuman HTRA2/Omiを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月26日 20時55分現在]
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HTRA2, Human, ELISA Kit, Quantikine (96 well) <Omi, ELISA Kit> |
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[在庫・価格 ：2025年04月26日 20時55分現在]
HTRA2, Human, ELISA Kit, Quantikine (96 well) <Omi, ELISA Kit>
文献数： 1
- 商品コード：DHTR20
- メーカー：RSD
- 包装：1kit
- 本製品は取扱中止になりました
| 説明文 | Genbank No：
27429
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| 法規制等 | |||
| 保存条件 | 法規備考 | ||
| 掲載カタログ |
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| 製品記事 | Quantikine Human HTRA2 ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (25 µL), Platelet-poor EDTA Plasma (25 µL), Platelet-poor Heparin Plasma (25 µL)
- Sensitivity
- 13.2 pg/mL
- Assay Range
- 31.3 - 2,000 pg/mL (Serum, Cell Culture Supernates, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma)
- Specificity
- Natural and recombinant HTRA2/Omi
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human HTRA2/Omi Immunoassay is a 4.5 hour solid phase ELISA designed to measure HTRA2 levels in cell culture supernates, serum, and platelet-poor plasma. It contains E. coil-expressed recombinant human HTRA2 and antibodies raised against the recombinant protein. Results obtained for naturally occurring human HTRA2 and recombinant human HTRA2 showed linear curves that were parallel to the standard curves obtained using the Quantikine Human HTRA2/Omi Immunoassay standards. These results indicate that this kit can be used to determine relative mass values for natural human HTRA2.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of a known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.
Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 284 | 582 | 1,108 | 280 | 567 | 1,089 |
| Standard Deviation | 15.7 | 15.9 | 28.6 | 25.1 | 41.3 | 65.3 |
| CV% | 5.5 | 2.7 | 2.6 | 9 | 7.3 | 6 |
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Recovery
The recovery of human VEGF spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 92 | 86-101 |
| Serum (n=4) | 91 | 80-111 |
| Platelet-poor EDTA Plasma (n=4) | 90 | 79-100 |
| Platelet-poor Heparin Plasma (n=4) | 89 | 81-95 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of HTRA2 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. 
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Additional Infomation
- Molecule Information
- HTRA2/Omi
- Aliases
- Omi; PRSS25
- Entrez Gene IDs
- 27429 (Human); 64704 (Mouse)
- Background
- HTRA2/Omi
- HTRA2/Omi is the mammalian homolog of the bacterial high temperature requirement protein (HTRA). HTRA2/Omi is a homotrimeric serine protease that localizes to the mitochondria and is processed to expose an N-terminal reaper-like motif similar to SMAC/Diablo. HTRA2/Omi is released from the mitochondria in response to apoptotic insult and can interact with the BIR2 or BIR3 domains of XIAP to relieve caspase-IAP inhibition.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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