Human Carbonic Anhydrase IX Quantikine ELISA Kit | hCA9 QKit
掲載日情報：2016/08/18 現在Webページ番号：111038
R&D Systems製のHuman Carbonic Anhydrase IXを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年05月18日 00時00分現在]
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Carbonic Anhydrase IX, Human, ELISA Kit, Quantikine (96well) |
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[在庫・価格 ：2024年05月18日 00時00分現在]
Carbonic Anhydrase IX, Human, ELISA Kit, Quantikine (96well)
文献数： 10
- 商品コード：DCA900
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
測定範囲：15.60 - 1000 pg/mL，感度：4.39 pg/mL，測定試料：Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Citrate Plasma (100 uL), Urine (100 uL)，種交差性：Human，検出方法：colorimetric，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96，別売品コントロール：QC24，測定時間：4.5 hours，測定因子：Carbonic Anhydrase IX/CA9 別名：CA9 Genbank No： 768 |
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| 保存条件 | 4℃ | 法規備考 | |
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| 製品記事 |
ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL), Citrate Plasma (100 µL), Urine (100 µL)
- Sensitivity
- 4.39 pg/mL
- Assay Range
- 15.6 - 1,000 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, Citrate Plasma, EDTA Plasma, Urine)
- Specificity
- Natural and recombinant human CA9
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human Carbonic Anhydrase IX Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human CA9 in cell culture supernates, serum, plasma, and urine. It contains NS0-expressed recombinant human CA9 ectodomain and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human CA9 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human CA9.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 166 | 338 | 673 | 166 | 333 | 647 |
| Standard Deviation | 6.36 | 7.19 | 20.5 | 10.5 | 21.6 | 39.7 |
| CV% | 3.8 | 2.1 | 3 | 6.3 | 6.5 | 6.1 |
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Recovery
The recovery of CA9 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=4) | 97 | 87-106 |
| Heparin Plasma (n=4) | 97 | 88-112 |
| Citrate Plasma (n=4) | 96 | 89-111 |
| EDTA Plasma (n=4) | 99 | 90-110 |
| Urine (n=4) | 101 | 89-114 |
| Cell Culture Media (n=4) | 110 | 103-114 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of CA9 were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- Carbonic Anhydrase IX/CA9
- Aliases
- CA9; G250; MN; RCC
- Entrez Gene IDs
- 768 (Human); 230099 (Mouse)
- Background
- Carbonic Anhydrases
- Carbonic anhydrases catalyze the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption. Human members of the CAs are secreted (CA6), membrane-bound (CA4, 9 to 12 and 14), cytosolic (CA1 to 3, 7, 8 and 13) or mitochondrial (CA5A and 5B). Some CAs may serve as markers for tumors and hypoxia. A subset of CAs lack CA activity due to point mutations but retain esterase function.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
A multi-analyte assay for the non-invasive detection of bladder cancer.
Chang , Myron, Dai , Yunfeng, Goodison , Steve, Rosser , Charles , Urquidi , Virginia
PLoS ONE 2012 7:e47469
Species: Human
Sample Type: Urine
Prognostic utility of pre-operative circulating osteopontin, carbonic anhydrase IX and CRP in renal cell carcinoma.
Br. J. Cancer 2012 107:1131-7
Species: Human
Sample Type: Plasma
Measuring carbonic anhydrase IX as a hypoxia biomarker: differences in concentrations in serum and plasma using a commercial enzyme-linked immunosorbent assay due to influences of metal ions.
Banks RE, Messenger MP, Selby PJ, Thompson D, Wind TC
Ann. Clin. Biochem. 2011 48:112-20
Species: Human
Sample Type: Serum
Myeloid Biomarkers Associated with Glioblastoma Response to Anti-VEGF Therapy with Aflibercept.
Bekele BN, Camphausen K, Chen A, Cloughesy T, de Groot JF, Deangelis L, Gilbert M, Heymach JV, Lassman A, Liu J, Mehta M, Piao Y, Prados M, Robins HI, Tran H, Wen PY, Wu HK, Yung W
Clin. Cancer Res. 2011 17:4872-4881
Species: Human
Sample Type: Plasma
High levels of carbonic anhydrase IX in tumour tissue and plasma are biomarkers of poor prognostic in patients with non-small cell lung cancer.
Ammadi RE, Bonnetaud C, Havet K, Hofman P, Hofman V, Ilie M, Mazure NM, Mograbi B, Mouroux J, Ortholan C, Pouyssegur J, Venissac N
Br. J. Cancer 2010 102:1627-35
Species: Human
Sample Type: Plasma
Expression of carbonic anhydrase 9, a potential intrinsic marker of hypoxia, is associated with poor prognosis in oesophageal squamous cell carcinoma.
Faried A, Fukai Y, Fukuchi M, Inose T, Kato H, Kimura H, Kuwano H, Masuda N, Miyazaki T, Nakajima M, Sohda M, Tanaka N
Br. J. Cancer 2008 99:1468-75
Species: Human
Sample Type: Cell Culture Supernates
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