Human TGF-beta 2 Quantikine ELISA Kit | "hTGF-b2 QKit, 2nd Gen"
掲載日情報：2016/08/18 現在Webページ番号：111022
R&D Systems製のHuman TGF-beta 2を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年04月30日 00時00分現在]
| 詳細 | 商品名 |
|
文献数 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
TGF-β2, Human, ELISA Kit, Quantikine (96 well) |
|
69 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
[在庫・価格 ：2024年04月30日 00時00分現在]
TGF-β2, Human, ELISA Kit, Quantikine (96 well)
文献数： 69
- 商品コード：DB250
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
測定範囲：31.20 - 2000 pg/mL，感度：7 pg/mL，測定試料：Cell Culture Supernates (125 uL), Serum (125 uL), EDTA Plasma (125 uL), Heparin Plasma (125 uL), Citrate Plasma (125 uL)，種交差性：Human，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC20，測定時間：4.5 hours，測定因子：TGF-beta 2 別名：BSC-1 cell growth inhibitor Genbank No： 7042 |
||||||
|---|---|---|---|---|---|---|---|
| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | 4℃ | 法規備考 | |||||
| 掲載カタログ |
|
||||||
| 製品記事 |
Helper T Cells Marker Antibodies |
||||||
| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
||||||
追加しました。
Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (125 µL), Serum (125 µL), EDTA Plasma (125 µL), Heparin Plasma (125 µL), Citrate Plasma (125 µL)
- Sensitivity
- 7 pg/mL
- Assay Range
- 31.2 - 2,000 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, Citrate Plasma, EDTA Plasma)
- Specificity
- Natural and recombinant human TGF-beta 2 and natural porcine TGF-beta 2.
- Interference
- Interference observed with 1 or more available related molecules.
追加しました。
Product Summary
The Quantikine Human TGF-beta 2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure activated TGF-beta 2 in cell culture supernates, serum, and plasma. The Quantikine Human TGF-beta 2 Immunoassay contains CHO cell-derived recombinant human TGF-beta 2 and antibodies raised against the recombinant protein. The assay has been shown to quantitate the recombinant factor accurately. Results obtained using human TGF-beta 2 showed linear curves that were parallel to the standard curves obtained using the recombinant TGF-beta 2 kit standards. These results indicate that this kit can be used to determine relative mass values for natural human TGF-beta 2.
追加しました。
Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 194 | 509 | 1,044 | 196 | 500 | 1,015 |
| Standard Deviation | 13.8 | 27 | 28.3 | 9.8 | 21.5 | 43.6 |
| CV% | 7.1 | 5.3 | 2.7 | 5 | 4.3 | 4.3 |
追加しました。
Recovery
To assess recovery of the assay, samples of various matrices were spiked with three concentrations of TGF-beta 2, activated, and measured in the assay.
| Sample Type | Average % Recovery | Range % |
| Serum (n=5) | 86 | 73-95 |
| Heparin Plasma (n=5) | 102 | 89-113 |
| Citrate Plasma (n=5) | 107 | 93-116 |
| EDTA Plasma (n=5) | 101 | 95-111 |
| Cell Culture Media (n=5) | 101 | 89-113 |
追加しました。
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of TGF-beta 2, activated and then serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
追加しました。
Additional Infomation
- Molecule Information
- TGF-beta 2
- Aliases
- TGFB2
- Entrez Gene IDs
- 7042 (Human); 21808 (Mouse); 397084 (Porcine)
- Background
- TGF-beta Family Ligands
- Transforming Growth Factor Beta 1, 2, and 3 (TGF-beta 1, TGF-beta 2, and TGF-beta 3) are highly pleiotropic cytokines that virtually all cell types secrete. TGF-beta molecules are proposed to act as cellular switches that regulate processes such as immune function, proliferation, and epithelial-mesenchymal transition. Targeted deletions of these genes in mice show that each TGF-beta isoform has some non-redundant functions: TGF-beta 1 is involved in hematopoiesis and endothelial differentiation; TGF-beta 2 affects development of cardiac, lung, craniofacial, limb, eye, ear, and urogenital systems; and TGF-beta 3 influences palatogenesis and pulmonary development. The full range of in vitro biological activities of TGF-beta 5 has not yet been explored. However, TGF-beta 1, TGF-beta 2, TGF-beta 3, and TGF-beta 5 have been found to be largely interchangeable in an inhibitory bioassay, and it is anticipated that TGF-beta 5 will show a spectrum of activities similar to the other TGF-beta family members. To date, the production of TGF-beta 5 has only been demonstrated in Xenopus.TGF-beta ligands are initially synthesized as precursor proteins that undergo proteolytic cleavage. The mature segments form active ligand dimers via a disulfide-rich core consisting of the characteristic 'cysteine knot'. TGF-beta signaling begins with binding to a complex of the accessory receptor betaglycan (also known as TGF-beta RIII) and a type II serine/threonine kinase receptor termed TGF-beta RII. This receptor then phosphorylates and activates a type I serine/threonine kinase receptor, either ALK-1 or TGF-beta RI (also called ALK-5). The activated type I receptor phosphorylates and activates Smad proteins that regulate transcription. Use of other signaling pathways that are Smad-independent allows for distinct actions observed in response to TGF-beta in different contexts.
追加しました。
Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Differential expression of cancer associated proteins in breast milk based on age at first full term pregnancy.
Arcaro KF, Browne EP, Kliethermes B, Qin W, Ruhlen RL, Sauter ER, Zhang K
BMC Cancer 2012 12:100
Species: Human
Sample Type: Milk
Mechanisms inducing low bone density in duchenne muscular dystrophy in mice and humans.
Bertini E, Bianchi ML, Capulli M, Carvello F, De Benedetti F, De Pasquale L, De Simone M, Del Fattore A, Ferrari S, Morandi L, Pierroz D, Rucci N, Rufo A, Teti A
J. Bone Miner. Res. 2011 26:1891-903
Species: Human
Sample Type: Serum
An autocrine TGF-beta/ZEB/miR-200 signaling network regulates establishment and maintenance of epithelial-mesenchymal transition.
Bert AG, Bracken CP, Drew PA, Farshid G, Goodall GJ, Gregory PA, Khew-Goodall Y, Lim YY, Lindeman GJ, Morris M, Roslan S, Shannon MF, Smith E, Wright JA, Wyatt L
Mol. Biol. Cell 2011 22:1686-98
Species: Canine
Sample Type: Cell Culture Supernates
Maternal country of origin, breast milk characteristics and potential influences on immunity in offspring.
Amoudruz P, Doumbo O, Doumbo S, Haileselassie Y, Holmlund U, Johansson MA, Kayentao K, Montgomery SM, Ongoiba A, Schollin J, Sverremark-Ekstrom E, Traore B
Clin. Exp. Immunol. 2010 162:500-9
Species: Human
Sample Type: Milk
Urinary proinflammatory cytokine response in renal transplant recipients with polyomavirus BK viruria.
Daniel V, Lahdou I, Naujokat C, Opelz G, Sadeghi M, Schnitzler P, Zeier M
Transplantation 2009 88:1109-16
Species: Human
Sample Type: Urine
Connexin43 knockdown accelerates wound healing but inhibits mesenchymal transition after corneal endothelial injury in vivo.
Dai P, Kinoshita S, Nakagami T, Nakano Y, Oyamada M, Takamatsu T
Invest. Ophthalmol. Vis. Sci. 2008 49:93-104
Species: Rat
Sample Type: Aqueous humor
Connective tissue growth factor as a mediator of intraocular fibrosis.
Barron E, Burton R, Chen Y, He S, Hinton DR, Khankan R, Oliver N, Ryan SJ, Zhu D
Invest. Ophthalmol. Vis. Sci. 2008 49:4078-88
Species: Human
Sample Type: Cell Culture Supernates
Potent inhibition of cicatricial contraction in proliferative vitreoretinal diseases by statins.
Arita R, Enaida H, Goto Y, Hafezi-Moghadam A, Hata Y, Ishibashi T, Kagimoto T, Kawahara S, Kita T, Miura M, Mochizuki Y, Nakao S
Diabetes 2008 57:2784-93
Species: Human
Sample Type: Vitreous Humor
Short communication: decreasing soluble CD30 and increasing IFN-gamma plasma levels are indicators of effective highly active antiretroviral therapy.
Daniel V, Huth-Kuhne A, Naujokat C, Opelz G, Sadeghi M, Susal C, Zimmermann R
AIDS Res. Hum. Retroviruses 2007 23:886-90
Species: Human
Sample Type: Plasma
Interferon-gamma differentially regulates TGF-beta1 and TGF-beta2 expression in human retinal pigment epithelial cells through JAK-STAT pathway.
Cherukuri KS, Detrick B, Hooks JJ, Kutty V, Nagineni CN
J. Cell. Physiol. 2007 210:192-200
Species: Human
Sample Type: Cell Culture Supernates
追加しました。
製品情報は掲載時点のものですが、価格表内の価格については随時最新のものに更新されます。お問い合わせいただくタイミングにより製品情報・価格などは変更されている場合があります。
表示価格に、消費税等は含まれていません。一部価格が予告なく変更される場合がありますので、あらかじめご了承下さい。