Human Attractin Quantikine ELISA Kit | hAttractin QKit
掲載日情報：2016/08/18 現在Webページ番号：111020
R&D Systems製のHuman Attractinを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月28日 16時55分現在]
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Human Attractin Quantikine ELISA Kit |
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[在庫・価格 ：2025年04月28日 16時55分現在]
Human Attractin Quantikine ELISA Kit
文献数： 0
- 商品コード：DATRN0
- メーカー：RSD
- 包装：1kit
- 本製品は取扱中止になりました
| 説明文 | Genbank No：
8455
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| 保存条件 | 法規備考 | ||
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| 製品記事 | Human Attractin Quantikine ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Saliva (10 µL), Urine (10 µL)
- Sensitivity
- 0.217 ng/mL
- Assay Range
- 0.781 - 50 ng/mL (Serum, Heparin Plasma, Saliva, Cell Culture Supernates, EDTA Plasma, Urine)
- Specificity
- Natural and recombinant human Attractin.
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human Attractin Immunoassay is a 4.5 hour solid phase ELISA designed to measure Attractin levels in cell culture supernates, serum, plasma, saliva, and urine. It contains CHO cell-expressed recombinant human Attractin and antibodies raised against the recombinant protein. Results obtained for naturally occurring human Attractin showed linear curves that were parallel to the standard curves obtained using the Quantikine Human Attractin Immunoassay standards. These results indicate that this kit can be used to determine relative mass values for natural human Attractin.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 4.16 | 12.7 | 26.2 | 4.61 | 13.7 | 27.5 |
| Standard Deviation | 0.132 | 0.324 | 0.893 | 0.498 | 1.1 | 1.54 |
| CV% | 3.2 | 2.6 | 3.4 | 10.8 | 8 | 5.6 |
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Recovery
The recovery of human Attractin spiked to levels throughout the range of the assay was evaluated
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 91 | 87-97 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Attractin were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- Attractin
- Aliases
- ATRN; DPPT-L; Mahogany Protein; MGCA
- Entrez Gene IDs
- 8455 (Human); 11990 (Mouse); 83526 (Rat)
- Background
- Attractin
- Attractin/ATRN, also known as DPPT-L, is an approximately 200 kDa transmembrane glycoprotein that shows dipeptidyl peptidase activity similar to DPPIV/CD26. Attractin is involved in a variety of processes including monocyte-T cell adhesion, axon myelination, melanocyte pigment synthesis, and energy homeostasis. Attractin is transiently upregulated during T cell activation before expression switches to the 175 kDa secreted isoform which is released into the circulation. Soluble Attractin is preferentially expressed by leukocytes and differentiating neurons. It blocks neurite formation and is elevated in the CSF of astrocytoma patients.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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