R&D Systems社のフローサイトメトリーに有用な抗CD1a抗体 抗CD1a抗体

R&D Systems社の抗CD1a抗体です。フローサイトメトリーに適用可能な製品を取りそろえています。
CD1aは、MHC（Major Histocompatibility Complex）様分子であるCD1ファミリーの膜貫通型糖タンパク質です。

※本製品は研究用です。研究用以外には使用できません。

Molecule Information: CD1a

Background: CD1a

Cluster of Differentiation 1a (CD1a) is a transmembrane glycoprotein in the CD1 family of MHC-like molecules. It is expressed by most mammals but not by mice or rats. Human CD1a is a 311 amino acid protein with a predicted molecular mass of 49 kDa. It binds to lipid and glycolipid antigens and presents them to T cells. Antigen-presenting cells, Langerhans cells, and thymocytes express CD1a, where it traffics between endosomes and plasma membrane lipid rafts. Heterodimerization with beta-2-Microglobulin is required for CD1a membrane trafficking, and CD1a binding to exogenous lipids results in CD1a protein stability. The activation of thymus-localized T cells during the development of Graves’ disease and Hashimoto’s thyroiditis may be initiated by CD1a expressed by CD83+ dendritic cells.

Applications & Data: Human CD1a Antibody (#MAB7076)

■Flow Cytometry

Detection of CD1a in MOLT‑4 Human Cell Line by Flow Cytometry.

MOLT‑4 human acute lymphoblastic leukemia cell line was stained with Mouse Anti-Human CD1a Monoclonal Antibody (# MAB7076, filled histogram) or isotype control antibody (# MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (# F0102B).

■Immunocytochemistry

CD1a in MOLT‑4 Human Cell Line.

CD1a was detected in immersion fixed MOLT‑4 human acute lymphoblastic leukemia cell line using Mouse Anti-Human CD1a Monoclonal Antibody (# MAB7076) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; # NL007) and counterstained with DAPI (blue). Specific staining was localized to surface and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.