Th1細胞の効率的な分化誘導試薬キット CellXVivo Human Th1 Cell Differentiation Kit

フローサイトメトリーによるTFN-γの発現量からのTh1細胞の分化の検証

Human peripheral blood naïve CD4+ T cells without (A, C) and with (B, D) a 5 day differentiation using the reagents included in the Human Th1 Cell Differentiation Kit. (A, B) The cells were stained with an APC-conjugated Mouse Anti-Human IFN-gamma Monoclonal Antibody and a PE-conjugated Mouse Anti-Human IL-4 Monoclonal Antibody. (C, D) The cells were stained with an APC-conjugated Mouse Anti-Human IFN-gamma Monoclonal Antibody and a PerCP-conjugated Mouse Anti-Human IL-17 Monoclonal Antibody. Control cultures were used to place the quadrants.

IFN-γ、IL-4、IL-17の発現量によるTh1細胞の分化の検証

Human peripheral blood naïve CD4+ T cells were cultured for 5 days using reagents included in the Human Th1 Cell Differentiation Kit. After fixation of cells in the wells, cytokine expression was determined using the Human IFN-gamma Quantikine ELISA Kit, the Human IL-4 Quantikine ELISA Kit, and the Human IL-17 Quantikine ELISA Kit.