Tリンパ球を抗原特異的に刺激できる感染症／腫瘍関連タンパク質ペプチド PepMix（部分ペプチドの混合物）

• 特長
• T細胞アッセイ用ポジティブコントロール
• T細胞アッセイ用ネガティブコントロール
• 感染症関連タンパク質
• 腫瘍関連タンパク質
• 使用文献例

特長

• ウイルスや腫瘍関連タンパク質がラインナップされています。
• 各部分ペプチドは15アミノ酸残基からなり，互いに11アミノ酸残基ずつオーバーラップさせながら抗原タンパク質の全長をカバーするように合成されています。
• 製品1 vialには各部分ペプチドが25μgずつ含まれています。
• CD4およびCD8陽性T細胞の免疫刺激に用いた場合，全長の抗原タンパク質と同等またはそれ以上の反応性を示します。
• CD4，CD8免疫応答を同時に刺激できます。
• 保存血液や末梢血単核球では，全長の抗原タンパク質を用いた場合よりも優れた反応性を示します。

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T細胞アッセイ用ポジティブコントロール

PepMix 品名	タンパク質	Accession No.	由　来	ペプチド数	商品コード
HCMVA, IE-1 〈55 kDa Immediate-Early Protein 1〉	VIE1_HCMVA	P13202	HCV	120	PM-IE1
HCMVA, IE-2 〈45 kDa Immediate-Early Protein 2〉	VIE2_HCMVA	P19893	HCV	143	PM-IE2
HCMVA, pp65 〈65 kDa Lower Matrix Phosphoprotein〉	PP65_HCMVA	P06725	HCV	138	PM-PP65

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T細胞アッセイ用ネガティブコントロール

PepMix 品名	タンパク質	Accession No.	由　来	ペプチド数	商品コード
HIV-1, Con B gag Motif	－	－	HIV	123	PM-HIV-CONB
Actin, Skeletal Muscle, Human	ACTS_HUMAN	P68133	Human	92	PM-ACTS
HLA Class Ⅰ, Ig-Like C1 〈HLA class Ⅰ Histocompatibility Antigen, A-36 α chain〉	1A36_HUMAN	P30455	Human	19	PM-C1
MOG, Human 〈Myelin-Oligodendrocyte Glycoprotein〉	MOG_HUMAN	Q16653	Human	29	PM-MOG

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感染症関連タンパク質

PepMix 品名	タンパク質	Accession No.	由来	ペプチド数	商品コード
Adenovirus (HAdV)
HAdV-3, Hexon Protein	HEX_ADE03	P36849	HAdV-3	234	PM-HAdV3
HAdV-5, Penton Protein	PEN3_ADE05	P12538	HAdV-5	140	PM-HAdV5
BKV (BK Polyomavirus)
BKV, Capsid Protein VP2 〈Minor Capsid Protein VP2〉	VP2_POVBK	P03094	BKV	85	PM-BKV-VP2
BKV, Coat Protein VP1 〈Major Capsid Protein VP1〉	VP1_POVBA	P14996	BKV	88	PM-BKV-VP1
BKV, Large T Antigen	LT_POVBA	P14999	BKV	170	PM-BKV-LTA
BKV, Small T Antigen	ST_POVBK	P03082	BKV	41	PM-BKV-STA
BKV, VP3 〈Minor Capsid Protein VP2, Isoform VP3〉	VP2_POVBK	P03094_2	BKV	56	PM-BKV-VP3
EBV (Epstein-Barr Virus)
EBV, BMLF1	EB2_EBVB9	Q04360	EBV (HHV-4)	117	PM-EBV-BMLF1
EBV, BMRF1	EAD_EBVB9	P03191	EBV (HHV-4)	99	PM-EBV-BMRF1
EBV, BRLF1	RTA_EBVB9	P03209	EBV (HHV-4)	149	PM-EBV-BRLF1
EBV, BZLF1	BZLF1_EBVB9	P03206	EBV (HHV-4)	59	PM-EBV-BZLF1
EBV, EBNA1	EBNA1_EBVB9	P03211	EBV (HHV-4)	158	PM-EBV-EBNA1
EBV, EBNA2	EBNA2_EBVB9	P12978	EBV (HHV-4)	119	PM-EBV-EBNA2
EBV, EBNA3a	EBNA3_EBVB9	P12977	EBV (HHV-4)	234	PM-EBV-EBNA3a
EBV, EBNA3b	EBNA4_EBVA8	Q1HVG4	EBV (HHV-4)	279	PM-EBV-EBNA3b
EBV, EBNA3c	EBNA6_EBVA8	Q69140	EBV (HHV-4)	265	PM-EBV-EBNA3c
EBV, EBNA-LP	EBNA5_EBVB9	Q8AZK7	EBV (HHV-4)	124	PM-EBV-EBNA-LP
EBV, gp340	GP350_EBVB9	P03200	EBV (HHV-4)	224	PM-EBV-GP340
EBV, LMP-1	LMP1_EBVB9	P03230	EBV (HHV-4)	94	PM-EBV-LMP1
EBV, LMP-2	LMP2_EBVB9	P13285	EBV (HHV-4)	122	PM-EBV-LMP2
HCV (Human Cytomegalovirus)
HCMVA, UL28	UL28_HCMVA	P16847	HCMVA (HHV-5)	92	PM-UL28
HCMVA, UL32	P100_HCMVA	P08318	HCMVA (HHV-5)	260	PM-UL32
HCMVA, UL36	UL36_HCMVA	P16767	HCMVA (HHV-5)	117	PM-UL36
HCMVA, UL40	UL40_HCMVA	P16780	HCMVA (HHV-5)	53	PM-UL40
HCMVA, UL48	DEN_HCMVA	P16785	HCMVA (HHV-5)	229＋229	PM-UL48
HCMVA, UL55	GB_HCMVA	P06473	HCMVA (HHV-5)	224	PM-UL55
HCMVA, UL82	PP71_HCMVA	P06726	HCMVA (HHV-5)	137	PM-UL82
HCMVA, UL94	UL16_HCMVA	P16800	HCMVA (HHV-5)	84	PM-UL94
HCMVA, UL99	TG11_HCMVA	P13200	HCMVA (HHV-5)	45	PM-UL99
HCMVA, UL103	UL103_HCMVA	P16734	HCMVA (HHV-5)	60	PM-UL103
HCMVA, UL151	Q68405_HCMVO	Q68405	HCMVA (HHV-5)	82	PM-UL151
HCMVA, UL153	Q68408_HCMV	Q68408	HCMVA (HHV-5)	67	PM-UL153
HCMVA, US3	US03_HCMVA	P09712	HCMVA (HHV-5)	44	PM-US3
HCMVA, US24	US24_HCMVA	P09700	HCMVA (HHV-5)	123	PM-US24
HCMVA, US29	US29_HCMVA	P09705	HCMVA (HHV-5)	113	PM-US29
HCMVA, US32	US32_HCMVA	P09708	HCMVA (HHV-5)	43	PM-US32
HIV (Human Immunodefficiency Virus)
HIV-1, Con B gag Motif	－	－	HIV	123	PM-HIV-CONB
IAV(Influenza A Virus)
Influenza A, Hemagglutinin, Brisbane (H1N1)	B0VX44_9INFA	B0VX44	Influenza A (A/Brisbane/59/2007 H1N1)	139	PM-INFA-HABris
Influenza A, Hemagglutinin, California (H1N1)	C3W5X2_9INFA	C3W5X2	Influenza A (A/California/08/2009 H1N1)	140	PM-INFA-HACal
Influenza A, Matrix Protein 1, Aichi (H3N2)	M1_I68A0	Q67157	Influenza A (A/Aichi/02/1968 H3N2)	110	PM-INFA_MP1
Influenza A, Matrix Protein 1, California (H1N1)	C3W5Z8_9INFA	C3W5Z8	Influenza A (A/California/08/2009 H1N1)	61	PM-INFA-MP1-H1N1
Influenza A, Matrix Protein 2, California (H1N1)	C3W5Z7_9INFA	C3W5Z7	Influenza A (A/California/08/2009 H1N1)	22	PM-INFA_MP2
Influenza A, Neuroamidase, California (H1N1)	C7FH14_9INFA	C7FH14	Influenza A (A/California/08/2009 H1N1)	115	PM-INFA_NA
Influenza A, Nucleocapsid Protein, Kitakyusyu (H3N2)	NCAP_I93A0	O91743	Influenza A (A/Kitakyushu/159/1993 H3N2)	122	PM-INFA_NP
RSV (Human Respiratory Syncytial Virus)
HRSVB, Fusion Glycoprotein F0	FUS_HRSVB	O36634	RSV	141	PM-RSV-FGF0
RSV, Nucleocapsid Protein N	NCAP_HRSVB	O42053	RSV	95	PM-HRSVB-NCPN
VZV (Varicella-Zoster Virus)
VSV, IE62	ICP4_VZVO	Q8AZM1	VZV (strain Oka vaccine)	325	PM-VZV-IE62	1 set
VSV, IE63	ICP22_VZVO	Q77NN7-1	VZV (strain Oka vaccine)	67	PM-VZV-IE63
Miscellaneous
HBV, Large Envelope Protein	HBSAG_HBVA4	P17101	HBV (Hepatitis B virus) (HBV-A)	98	PM-HBV-LEP
HHV6, U54	U54_HHV6B	Q9QJ29	HHV-6B (Human herpesvirus-6B)	112	PM-HHV6-U54
MP65, Candida	Q9HEP1_CANAL	Q9HEP1	Candida albicans (Yeast)	92	PM-CAND-MP65
YFV, NS4B	POLG_YEFV1	P03314	YFV (Yellow fever virus)	60	PM-YF-NS4B

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腫瘍関連タンパク質

PepMix 品名	タンパク質	Accession No.	由来	ペプチド数	商品コード
Breast / Prostate
Mammaglobin A, Human	SG2A2_HUMAN	Q13296	Human	21	PM-MamA
NY-ESO-1, Human 〈Cancer / Testis Antigen 1〉	CTG1B_HUMAN	P78358	Human	43	PM-NYE
PSA, Human	KLK3_HUMAN	P07288	Human	63	PM-PSA
SSX2, Human	SSX2_HUMAN	Q16385	Human	45	PM-SSX2
Epithelia
CEA, Human	CEAM5_HUMAN	P06731	Human	173	PM-CEA
Claudin-6, Human	CLD6_HUMAN	P56747	Human	53	PM-CLD6
Stromelysin-3, Human 〈MMP-11〉	MMP11_HUMAN	P24347	Human	121	PM-MMP11
Hematopoietic Tumors
c-Myc, Human	MYC_HUMAN	P01106	Human	107	PM-cMyc
Melanoma
gp100, Human 〈Melanocyte Protein Pmel 17〉	PMEL_HUMAN	P40967	Human	163	PM-GP100
OIP4, Human 〈Prame〉	PRAME_HUMAN	P78395	Human	125	PM-OIP4
MAGEA1, Human	MAGA1_HUMAN	P43355	Human	75	PM-MAGEA1
MAGEA3, Human	MAGA3_HUMAN	P43357	Human	76	PM-MAGEA3
MAGEA4, Human	MAGA4_HUMAN	P43358	Human	77	PM-MAGEA4
Melan-A, Human 〈MART-1〉	MAR1_HUMAN	Q16655	Human	27	PM-MelA
Vaccinia Virus
VACV, MVA018L	VHR2_VACCA	P68598	VACV (strain Ankara)	35	PM-MVA-018L
VACV, MVA074R	O57196_9POXV	O57196	VACV (strain Ankara)	106	PM-MVA-074R
VACV, MVA093L	O57206_9POXV	O57206	VACV (strain Ankara)	79	PM-MVA-093L
VACV, MVA105L	CAHH_VACCA	O57211	VACV (strain Ankara)	74	PM-MVA-105L
VACV, MVA121L	O57223_9POXV	O57223	VACV (strain Ankara)	112＋111	PM-MVA-121L
VACV, MVA189R	O57265_9POXV	O57265	VACV (strain Ankara)	45	PM-MVA-189R
Wilms Tumor1
WT1, Human 〈WT33〉	WT1_HUMAN	P19544	Human	110	PM-WT1
Miscellaneous
Cyclin B1, Human	CCNB1_HUMAN	P14635	Human	106	PM-CYC_B1
ErbB2, Intracellular Domain, Human	ERBB2_HUMAN	P04626	Human	146	PM-ERB_ICD
Histone H1.2, Human	H12_HUMAN	P16403	Human	51	PM-H1
Histone H4, Human	H4_HUMAN	P62805	Human	23	PM-H4
p53, Human	P53_HUMAN	Q2XN98	Human	96	PM-p53
SOX-2, Human	SOX2_HUMAN	P48431	Human	77	PM-SOX-2
Survivin, Pongo abelii	BIRC5_PONAB	Q5RAH9	Pongo abelii (Sumatran orangutan)	33	PM-Survivin
TARP, Human	A2JGV3_HUMAN	A2JGV3	Human	12	PM-TARP
TRP-2, Human	TYRP2_HUMAN	P40126	Human	127	PM-TRP-2
Tyrosinase, Human	TYRO_HUMAN	P14679	Human	130	PM-Tyr
Tyrosinase, Mouse	TYRO_MOUSE	P11344	Mouse	117	PM-MM-Tyr
WRKY47, Arabidopsis	Q9ZS17_ALOMY	Q9ZS17	Arabidopsis thaliana (Mouse-ear cress)	120	PM-WRK

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使用文献例

■感染症関連タンパク質

• Shelley Waters, et.al., Noncoding RNA. (2020) - PMID: 33353107
  "Human Cytomegalovirus-Encoded microRNAs Can Be Found in Saliva Samples from Renal Transplant Recipients."
• Daniela Di Blasi, et.al., Journal of Immunological Methods (2020) - PMID: 33152332
  "Guidelines For Analysis of Low-Frequency Antigen-Specific T Cell Results: Dye-Based Proliferation Assay vs 3H-Thymidine Incorporation."
• Kateřina Roubalová, et.al., Immunol Lett. (2020) - PMID: 33031870
  "Antigenic Competition in the Generation of Multi-Virus-Specific Cell Lines for Immunotherapy of Human Cytomegalovirus, Polyomavirus BK, Epstein-Barr Virus and Adenovirus Infection in Haematopoietic Stem Cell Transplant Recipients."
• Marta Lepore, et.al., Transplant Infectious Disease (2020) - PMID: 33070461
  "Functional Immune Monitoring of BK Virus And Donor‐Specific T‐cell Effector Immune Responses to Guide Treatment Decision‐Making After Kidney Transplantation; an Illustrative Case Report And Literature Review."
• Nina Körber, et.al., Journal of Translational Medicine (2020) - PMID: 32552697
  "Evaluation of T-Activated Proteins as Recall Antigens to Monitor Epstein–Barr Virus and Human Cytomegalovirus-Specific T Cells in a Clinical Trial Setting."
• Carolina Chiale, et.al., Vaccines (2020) - PMID: 32517032
  "Modified Alphavirus-Vesiculovirus Hybrid VaccineVectors for Homologous Prime-Boost Immunotherapyof Chronic Hepatitis B."

■腫瘍関連タンパク質

• Premal Lulla, et.al., Blood (2020) - PMID: 33270816
  "Clinical Effects of Administering Leukemia-Specific Donor T Cells to Patients With AML/MDS Post-Allogeneic Transplant."
• Nandakumar Packiriswamy, et.al., Leukemia (2020) - PMID: 32327728
  "Oncolytic Measles Virus Therapy Enhances Tumor Antigen-Specific T-Cell Responses in Patients With Multiple Myeloma."
• Maria O.López-Oliva, et.al., Clinical Immunology (2020) - PMID: 31891764
  "Pre-Transplant Assessment of pp65-Specific CD4 T Cell Responses Identifies CMV-Seropositive Patients Treated With rATG at Risk of Late Onset Infection."
• Carly M. Bliss, et.al., Molecular Therapy: Methods & Clinical Development (2019) - PMID: 31934599
  "Targeting Antigen to the Surface of EVs Improves the In Vivo Immunogenicity of Human and Non-human Adenoviral Vaccines in Mice."
• Qi Xu, et.al., International Journal of Cancer (2019) - PMID: 31584185
  "Evaluation of Newcastle Disease Virus Mediated Dendritic Cell Activation and Cross‐Priming Tumor‐Specific Immune Responses Ex Vivo."
• Anna Przybyla, et.al., Cancer Immunology, Immunotherapy (2019) - PMID: 30783693
  "Natural T Cell Autoreactivity to Melanoma Antigens: Clonally Expanded Melanoma-Antigen Specific CD8 + Memory T Cells Can be Detected in Healthy Humans."
• Jelle de Wit, et.al., Journal of Virology (2019) - PMID: 30626672
  "The Human CD4 T Cell Response Against Mumps Virus Targets a Broadly Recognized Nucleoprotein Epitope."
• Erika J Crosby, et.al., Clinical Cancer Research (2019) - PMID: 30635338
  "Vaccine-Induced Memory CD8+ T Cells Provide Clinical Benefit in HER2 Expressing Breast Cancer: a Mouse to Human Translational Study."
• Joseph A. Impellizeri, et.al., Journal of Translational Medicine (2018) - PMID: 30537967
  "Tel-eVax: a Genetic Vaccine Targeting Telomerase for Treatment of Canine Lymphoma."
• Loosdrecht, et.al., Cancer Immunol Immunother. (2018) - PMID: 30039426
  "A Novel Allogeneic Off-The-Shelf Dendritic Cell Vaccine for Post-Remission Treatment of Elderly Patients with Acute Myeloid Leukemia."

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