各種蛍光色素を包含するミクロスフェア（Bangs Laboratories社） 蛍光ミクロスフェア（Fluorescent Microspheres）

• 製品の種類
• 蛍光ミクロスフェア
• 蛍光磁性ミクロスフェア
• ストレプトアビジン（SA）修飾蛍光ミクロスフェア
• 蛍光色素の特長
• 関連製品

製品の種類

• 蛍光ミクロスフェア
• 蛍光磁性ミクロスフェア
• ストレプトアビジン（SA)コート蛍光ミクロスフェア

目次に戻る

蛍光ミクロスフェア

• 基材：ポリスチレン

製品群	表面修飾	蛍光色素	平均粒子径（μm）	商品コード
Non-functionalized Microspheres	なし	Plum Purple	0.50	FSPP003
			1.00	FSPP004
			2.00	FSPP005
		Envy Green	1.00	FSEG004
			4.00	FSEG006
			10.0	FSEG008
		Dragon Green	0.05	FSDG001
			0.20	FSDG002
			0.50	FSDG003
			1.00	FSDG004
			2.00	FSDG005
			4.00	FSDG006
			7.5	FSDG007
			15.0	FSDG009
			＞25.0	FSDG011
		Suncoast Yellow	0.20	FSSY002
			7.5	FSSY007
		Flash Red	0.05	FSFR001
			0.20	FSFR002
			0.50	FSFR003
			1.00	FSFR004
			2.00	FSFR005
			4.00	FSFR006
			7.5	FSFR007
Carboxylate Microspheres	-COOH	Glacial Blue	0.20	FCGB003
			1.0	FCGB006
			5.0	FCGB008
		Dragon Green	0.05	FCDG001
			0.10	FCDG002
			0.20	FCDG003
			0.40	FCDG004
			0.50	FCDG005
			1.0	FCDG006
			5.0	FCDG008
			10.0	FCDG009
			15.0	FCDG011
		Sunset Yellow	1.0	FCSY006
			2.0	FCSY007
		Envy Green	1.0	FCEG006
			5.0	FCEG008
		Surf Green	0.20	FCSG003
		Europium Chelate	0.10	FCEU001
			0.20	FCEU002
			0.30	FCEU003
		Europium Chelate-COOH Sampler Pack	0.10 / 0.20 / 0.30	21960
		Flash Red	0.05	FCFR001
			0.20	FCFR003
			0.40	FCFR004
			0.50	FCFR005
			1.0	FCFR006
			5.0	FCFR008

参考文献

Fluorescent Polystyrene

1. Lee, M., et al., "Construction and calibration of an optical trap on a fluorescence optical microscope.", Nat. Protocols, 2, 3226～3238 (2007). [PMID：18079723]
2. Mattheyses, A., et al., "Imaging with total internal reflection fluorescence microscopy for the cell biologist.", J. Cell Sci., 123, 3621～3628 (2010). [PMID：20971701 ]
3. Sun, Z., et al., "Mechanical properties of the interaction between fibronectin and α5β1-integrin on vascular smooth muscle cells studied using atomic force microscopy.", Am. J. Physiol. Heart Circ. Physiol., 289(6), H2526-H2535 (2005).
4. Ogilvie, J., et al., "Fourier-transform coherent anti-Stokes Raman scattering microscopy.", Optics Letters, Optical Society of America, 31(4), 480～482 (2006). [PMID：16496893]
5. Chen, X., et al., "Ultra-fast bright field and fluorescence imaging of the dynamics of micrometer-sized objects.", Review of Scientific Instruments, 84, 063701 (2013). [PMID：23822346]
6. Yang Z., et al., "Development of far-red and ultraviolet digital frequency-domain flow cytometry systems.", IEEE Journal of Selected Topics in Quantum Electronics, 23(3), 59～63.(Flash Red & Glacial Blue microspheres to determine detection sensitivity in a Far-Red and UV modified instrument) (2017).

Fluorescent Carboxyl Polystyrene

1. Adams, EW., et al., "Encoded fiber-optic microsphere arrays for probing protein-carbohydrate interactions.", Angew. Chem. Int. Ed. Engl., 10;42(43), 5317～5320. (Cat. 235, QuantumPlex Starfire Red 4.4μm COOH) (2003). [PMID：14613164]
2. Shapiro, K., et al., "Detection of Toxoplasma gondii oocysts and surrogate microspheres in water using ultrafiltration and capsule filtration.", Water Research, 44(3), 893～903. (10.35μm Dragon Green Carboxyl, 8.6μm Glacial Blue Carboxyl) (2010).
3. Pei, X., et al., "Multiplexed Detection of Attomoles of Nucleic Acids Using Fluorescent Nanoparticle Counting Platform.", Anal. Chem., 10.1021/acs.analchem.7b04551 (~200nm carboxylated Glacial Blue, Dragon Green & Envy Green beads) (2017). [PMID：29226673]

Europium chelate nanoparticles

1. Liang, R-L., et al., "Rapid and sensitive lateral flow immunoassay method for determining alpha fetoprotein in serum using europium (III) chelate microparticles-based lateral flow test strips.", Anal. Chim. Acta, 891, 277～283 (2015) . [PMID：26388387]
2. Soukka, T., et al., "Supersensitive time-resolved immunofluorometric assay of free prostate-specific antigen with nanoparticle label technology.", Clin. Chem., 47(4), 1269～1278 (2001).[PMID：11427459]
3. Xia, X., et al., "A highly sensitive nanoparticle-based lateral flow immunoassay for detection of chloramphenicol residue.", Anal. Bioanal. Chem., 405(23), 7541～7544 (2013). [PMID：23934393]
4. Yeo, SJ., et al., "Improvement of a rapid diagnostic application of monoclonal antibodies against avian influenza H7 subtype virus using Europium nanoparticles.", Scientific Reports, 7, 7933 (2017). [PMCID：PMC55541]
5. Tuan, Bao D., et al., "Rapid Detection of Avian Influenza Virus by Fluorescent Diagnostic Assay using an Epitope-Derived Peptide Theranostics.", 7(7), 1835～1846 (2017).
6. Tang, Y., et al., "Advantage of Eu3+ -Doped Polystyrene Microspheres Compared with Colloidal Gold Used in Immunochromatographic Assays for the Detection of Melamine in Milk.", J. Food Sci. Mar., 82(3), 694～697 (200nm Eu chelate microspheres) (2017). [PMCID：PMC54792]
7. Do Thi Hoang Kim DT., et al., "Development of a novel peptide aptamer-based immunoassay to detect Zika virus in serum and urine.", Theranostics, 8(13), 3629.(FCEU001, 0.1μm Euroipium chelate COOH nanoparticles) (2018). [PMCID：PMC6037026]
8. Yu, ST, et al., "Clinical evaluation of rapid fluorescent diagnostic immunochromatographic test for influenza A virus (H1N1).", Scientific reports, 8(1), 13468.(200nm Eu chelate nanoparticles coated with anti-influenza A nucleoprotein) (2018). [PMID：30194330]

目次に戻る

蛍光磁性ミクロスフェア

• 製品群：Carboxylate Microspheres

基材	表面修飾	蛍光色素	平均粒子径（μm）	商品コード
Classical Magnetic Polymer	-COOH	Dragon Green	0.9	MCDG001
		Flash Red	0.9	MCFR001
Encapsulated Magnetic Polymer	-COOH	Glacial Blue	1.63	MEGB002
		Dragon Green	0.86	MEDG001
			1.63	MEDG002
		Suncoast Yellow	1.63	MESY002
		Flash Red	0.86	MEFR001
			1.63	MEFR002
COMPEL Magnetic -COOH modified	-COOH	Glacial Blue	3	UMGB001
			6	UMGB002
			8	UMGB003
		Envy Green	3	UMEG001
		Dragon Green	3	UMDG001
			6	UMDG002
			8	UMDG003
		Flash Red	3	UMFR001
			6	UMFR002
			8	UMFR003

参考文献

Fluorescent Magnetic Polymer

1. Hinds, K., et al., "Highly efficient endosomal labeling of progenitor and stem cells with large magnetic particles allows magnetic resonance imaging of single cells.", Blood Aug., 102 (3), 867～872 (2003). [PMID：]
2. Shapiro, E M., et al., "Sizing it up: Cellular MRI using micron-sized iron oxide particles. Magn.", Reson. Med., 53, 329～338 (2005).
3. Rohani, R., et al., "In Vivo Cellular MRI of Dendritic Cell Migration Using Micrometer-Sized Iron Oxide (MPIO) Particles.", Mol. Imaging Biol., 13, 679 (2011) . [PMID：20803172]
4. Slotkin,J., et al., "Cellular Magnetic Resonance Imaging: Nanometer and Micrometer Size Particles for Noninvasive Cell Localization.," Neurotherapeutics, 4(3), 428～433 (2007). [PMID：17599708]
5. Tarulli E., et al., "Effectiveness of micron-sized superparamagnetic iron oxide particles as markers for detection of migration of bone marrow-derived mesenchymal stromal cells in a stroke model.", J. Magn. Reson. Imaging. Jun., 37(6) ,1409～1418. (MCDG001, 0.9μm Dragon Green Magnetic Classical, 60% mag) (2013). [PMID：23712844]

目次に戻る

ストレプトアビジン（SA）修飾蛍光ミクロスフェア

表面修飾	蛍光色素	平均粒子径（μm）	商品コード
Streptavidin	Dragon Green	0.20	CFDG001
		0.40	CFDG002
		0.50	CFDG003
		1.00	CFDG004
	Flash Red	0.40	CFFR002
		1.00	CFFR004

目次に戻る

蛍光色素の特長

目次に戻る

関連製品

StarLight Calibration Slides

品名		包装	商品コード
StarLight Calibration Slide	Glacial Blue	1 slide	SL1GB
	Dragon Green		SL1DG
	Envy Green		SL1EG
	Flash Red		SL1FR
	Slide 4-Pack	4 slides	SL04K

Fluorescent Intensity Standard Kit

品名	適合フィルターセット	包装	商品コード
Dragon Green Intensity Standard	フルオレセイン	1 ml	DG06M
Flash Red Intensity Standard	Cy5（蛍光顕微鏡）PE-Cy5 / APC（フローサイトメーター）	1 ml	FR06M

目次に戻る