R&D Systems社 キナーゼ活性測定キット Universal Kinase Activity Kit
掲載日情報:2012/06/01 現在Webページ番号:6224
追加しました。
R&D Systems now offers a Universal Kinase Activity Kit (Catalog # EA004) for assessing kinase activity in vitro . The majority of kinases use ATP as the phosphate donor, transferring the terminal phosphate group of ATP to the substrate protein and producing ADP as a by-product. The Universal Kinase Activity Kit is an ADP-based, phosphatase-coupled kinase assay that utilizes CD39L2/ENTPD6 as a coupling phosphatase. CD39L2 releases the beta-phosphate from ADP and the released inorganic phosphate is detected using malachite green reagents. The amount of inorganic phosphate released is proportional to the amount of ADP generated during the kinase reaction and reflects the kinetics of the reaction.
Features of the Universal Kinase Activity Kit
- Applicable for all kinase reactions that produce ADP
- Non-radioactive
- No labeling, conjugation, or antibody is used
- No time-consuming separation steps (30 to 60 minute assay)
- Quantitative
- Amenable to high-throughput analysis

Measuring PKA C beta Specific Activity. The activity of Recombinant Human PKA C beta (Catalog # 4596-KS) was assessed using the Universal Kinase Activity Kit (Catalog # EA004). PKA C beta catalyzed the transfer of phosphate from ATP to a PKA peptide substrate. Inorganic phosphate was generated from ADP with the phosphatase CD39L2 (0.1 μg/50 μL) and the levels were measured using malachite green detection reagents. The specific activity (SA) of PKA C beta was 670 pmol/min/μg. This was calculated using the slope (0.908 OD/μg), the phosphate conversion factor (CF; 3505 pmol/OD), the reaction time (t , 10 minutes), and the coupling rate (r, 0.475), according to the equation:
[在庫・価格 :2025年05月11日 00時00分現在]
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[在庫・価格 :2025年05月11日 00時00分現在]
Universal Kinase Activity Kit
文献数: 4
- 商品コード:EA004
- メーカー:RSD
- 包装:1kit
- 価格:¥125,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:医薬用外劇物
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