抗Blue Marker (6F4-F6)抗体 | Anti- Blue Marker (6F4-F6) antibody

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	Western Blot: Blue Marker Antibody (6F4-F6) [NBP2-33376] - Analysis using the HRP conjugate of NBP2-33376. Precision Plus Dual Color molecular weight markers (1 and 2 ul) were separated by SDS-PAGE on a 4-14% gel and transferred to PVDF membrane. The membrane was incubated with HRP conjugated Blue Marker Antibody at 1 ug/ml for 30 min. After washing, the membrane was exposed to ECL and the blue markers detected with a 5 second exposure.
	Western Blot: Blue Marker Antibody (6F4-F6) [NBP2-33376] - The indicated amounts of the BioRad Precision Plus Dual Color and All Blue protein standards were separated by SDS-PAGE on a 4-15% gel, transferred to PVDF membrane. The membrane was blocked for 1 hour in 5% non-fat milk in TBST and then incubated with 1.0 ug/mL of Anti-blue antibody and a 1:10,000 dilution of Anti-mouse HRP in 1% non-fat milk TBST for 1 hour. After washing the membrane was processed for chemiluminescent detection.
	Western Blot: Blue Marker Antibody (6F4-F6) [NBP2-33376] - Highly specific to blue prestained markers. Does not cross react with whole cell proteins of different species. Whole cell lysate from various cell lines of human, mouse, rat and hamster origin were separated on a 4-15% gel by electrophoresis and transferred to PVDF. The membrane was stained with Amido black to show the presence of protein from all lysates. After blocking in 5% non-fat milk, the membrane was incubated with Anti-Blue at 1 ug/ml. After washing and incubation with Anti-mouse HRP the blot was incubated in ECL reagent and imaged. Note that only the blue molecular weight markers were detected with the Anti-Blue antibody demonstrating its high specificity and suitability for addition to your Western blot procedure.
	Western Blot: Blue Marker Antibody (6F4-F6) [NBP2-33376] - Works with all prestained blue markers. Prestained molecular weight markers (2 and 5 ul) from three suppliers, BioRad, Invitrogen and GenDepot were separated by electrophoresis on a 4-15% gel and transferred to PVDF membrane. After blocking in 5% non-fat milk the blot was probed with 1 ug/ml Anti-Blue, washed and incubated with an Anti-mouse HRP conjugated secondary antibody followed by ECL incubation. The composite figure shows the visible image of the markers on the blot and its corresponding Western blot image. Note that only the blue protein markers from each supplier are detected by Western blot.
	Western Blot: Blue Marker Antibody (6F4-F6) [NBP2-33376] - Analysis using the HRP conjugate of NBP2-33376. Detection of the location of each of the protein standards is hand marked on the X-ray film or image after exposure. This can lead to errors and inaccuracy in determining the molecular weight and identity of your target protein (Tubulin and Actin are shown as examples in these images). By using the Anti-Blue antibody in conjunction with your target protein antibodies, you can image the complete molecular weight standard and your target protein at the same time.

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Background

The Blue Marker Antibody (6F4-F6) is a unique antibody that specifically detects the blue prestained molecular weight markers from different vendors. With the Blue Marker Antibody there is no longer a need to manually mark your protein marker bands and guess how accurately the marker bands were charted. The Blue Marker Antibody visualizes the markers together on the same western blot as your protein of interest. This antibody has outstanding specificity, it does not interfere with the detection by other antibodies and does not cross-react with whole cell proteins of different species.

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価格

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標識済み抗体価格

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