抗TNF-alpha (6N1E7)抗体 | Anti- TNF-alpha (6N1E7) antibody

• Image
• Background
• 価格
• 標識済み抗体価格

Image

	Western Blot: TNF-alpha Antibody (6N1E7) - Azide Free [NBP2-27224] - Analysis of TNF alpha recombinant protein using TNF alpha antibody at 3 ug/ml. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
	Biological Strategies Validation. Flow Cytometry: TNF-alpha Antibody (6N1E7) - Azide Free [NBP2-27224] - Human PBMC were stimulated with PMA (10 ng/ml) and Ionomycin (500 ng/ml) for 5h in presence of Brefeldin A. At the end of stimulation, cells were surface stained with before testing with by intracellular staining. Fixed and permeabilized cells were incubated with 10 ul (0.5 ug) of TNF antibody (or ) for 30 minutes on ice. Lymphocyte population was gated for analysis to show TNF alpha positive T cells.
	Blocking/Neutralizing: TNF-alpha Antibody (6N1E7) - Azide Free [NBP2-27224] - Validation of inhibitory effects of TNF alpha antibody on TNF alpha activity in the cell line. NF-kB/SEAPorter cells were plated in 96-well plate at 5 x 10^4 cells per well for 16 h. Cells were pre-incubated with TNF alpha antibody at 10 and 20 ug/ml for 1 hr, then treated with 10 ng/ml TNF alpha or 10 ng/ml PMA. After 24h, the secreted alkaline phosphatase (SEAP) was analyzed using the SEAPorterAssay Kit.

目次に戻る

Background

Tumor necrosis factor-alpha, TNF alpha, also in the past named cachexin or macrophage cytotoxic factor (MCF), is an important regulatory cytokine involved in the inflammatory process. Human TNF alpha is a non-glycosylated protein of about 17kDa. Involvement as a modulatory cytokine in a variety of diverse pathways besides and including inflammation, TNF alpha is also part of the regulatory processes in tumor metastases, rheumatoid arthritis, and septic shock. Binding of TNF alpha to receptors may lead to apoptosis, differentiation or proliferation depending on the specific receptor engaged, of which TNFRI is identified as the major receptor. TNF alpha signals through major pathways such as NF-kB, JNk and MAPk. Activated macrophages serve as a major source of TNF alpha which is also secreted by T cells, Nk cells, certain tumor cells or cell lines. Perturbed or dysfunctional TNF-alpha regulation is closely associated with a spectrum of autoimmune inflammatory diseases such as Rheumatoid Arthritis, Crohn's disease, MS and Psoriasis. Specific blockage of TNF alpha by antibodies such as Remicade and Humira has been tremendously successful as therapeutic modalities for such autoimmune diseases.

目次に戻る

価格

目次に戻る

標識済み抗体価格

目次に戻る