抗p62/SQSTM1 (5H7E2)抗体 | Anti- p62/SQSTM1 (5H7E2) antibody

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	Genetic Strategies Validation. Western Blot: p62/SQSTM1 Antibody (5H7E2) [NBP2-23490] - Western blot analysis using p62/SQSTM1 mAb in (1) HEK293 and (2) SQSTM1 hIgGFc transfected HEK293 cell lysate.
	Immunohistochemistry: p62/SQSTM1 Antibody (5H7E2) [NBP2-23490] - p62/SQSTM1 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
	Western Blot: p62/SQSTM1 Antibody (5H7E2) [NBP2-23490] - WB detection of p62 protein in lysates of Hela, Jurkat, THP-1, COS7, and SK-BR-3 cell lines using p62/SQSTM1 antibody clone 5H7E2. The antibody detected a specific band of p62 at ~62kDa position.
	Western Blot: p62/SQSTM1 Antibody (5H7E2) [NBP2-23490] - WB analysis of p62 protein in lysates of HEK293, A549, MCF-7, and HepG2 cell lines using p62/SQSTM1 antibody clone 5H7E2. The antibody detected a specific band at ~62kDa position.
	Immunohistochemistry: p62/SQSTM1 Antibody (5H7E2) [NBP2-23490] - IHC analysis of paraffin-embedded bladder cancer tissues using SQSTM1 mouse mAb with DAB staining.
	Flow Cytometry: p62/SQSTM1 Antibody (5H7E2) [NBP2-23490] - Flow cytometry analysis of HEK293 cells using SQSTM1 mouse mAb (green) and negative control (red).
	Western Blot: p62/SQSTM1 Antibody (5H7E2) [NBP2-23490] - Western blot analysis using p62/SQSTM1 mAb against human SQSTM1 recombinant protein. (Expected MW is 39.1 kDa)
	Immunohistochemistry: p62/SQSTM1 Antibody (5H7E2) [NBP2-23490] - IHC analysis of paraffin-embedded human cervical cancer tissues using p62/SQSTM1 mouse mAb with DAB staining.

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Background

p62/SQSTM1 (sequestosome 1) is ubiquitously-expressed cytoplasmic/adapter protein conserved in vertebrates and it can be induced by proteasomal inhibitor PSI, PGJ2/prostaglandin J2 as well as phorbol 12-myristate 13-acetate (PMA). SQSTM1 has the ability of ubiquitin binding as well as regulating NFkB1 activation by TNF-alpha, NGF (nerve growth factor) and interleukin-1. SQSTM1 acts as an adapter that mediates the interaction between TRAF6 and CYLD, and SQSTM1-TRAF6 interaction leads to K63-linked polyubiquitination of TRAF6 followed by subsequent activation of NFkB pathway. SQSTM1 plays a role in TITIN/TTN downstream signaling in muscle cells and regulate signaling cascades through ubiquitination. SQSTM1 is essential both for formation and autophagic degradation of polyubiquitin-containing bodies which are known as aggresome-like induced structures (ALIS) and SQSTM1 connects ALIS to the autophagic machinery via direct interaction with MAP1 LC3 family members. Moreover, while autophagy modulates SQSTM1 protein levels, SQSTM1 suppresses autophagy via activation of mTORC1. SQSTM1 interacts with KEAP1, which is a cytoplasmic inhibitor of NRF2, a key transcription factor involved in cellular responses to oxidative stress. SQSTM1 functions as a signaling hub for various signal transduction pathways, apoptosis, cell differentiation, apoptosis, immune response, regulation of K+ channels and Nrf2 activation, and its dysregulation is associated with Paget disease of bone and tumorigenesis.

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