抗PGC1 alpha抗体 | Anti- PGC1 alpha antibody

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	Western Blot: PGC1 alpha Antibody [NBP1-04676] - Total protein from human adipose and skeletal muscle tissue, HeLa and A431 cells lines was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-PGC1-alpha in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence
	Immunohistochemistry-Paraffin: PGC1 alpha Antibody [NBP1-04676] - IHC analysis of PGC-1 alpha in mouse prostate using DAB with hematoxylin counterstain.
	Immunohistochemistry-Paraffin: PGC1 alpha Antibody [NBP1-04676] - IHC analysis of a formalin-fixed paraffin-embedded tissue section of mouse prostate using rabbit polyclonal PGC1 alpha antibody at 1:200 dilution. The antibody generated an expected strong nuclear (punctate in some cells) and a weak cytoplasmic staining in the glandular cells lining of tubule-alveolar gland.
	Western Blot: PGC1 alpha Antibody [NBP1-04676] - Total protein from murine skeletal muscle tissue. This image was submitted via customer Review.
	Immunocytochemistry/Immunofluorescence: PGC1 alpha Antibody [NBP1-04676] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-PGC-1 alpha [NBP1-04676] at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
	Immunocytochemistry/Immunofluorescence: PGC1 alpha Antibody [NBP1-04676] - NIH-3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-PGC1 alpha at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
	Immunocytochemistry/Immunofluorescence: PGC1 alpha Antibody [NBP1-04676] - PC12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-PGC1 alpha at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
	Flow (Intracellular): PGC1 alpha Antibody [NBP1-04676] - An intracellular stain was performed on HepG2 cells with NBP1-04676 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).
	Western Blot: PGC1 alpha Antibody [NBP1-04676] - PGC1 alpha expression in lysates from Siberian hamster using anti-PGC1 alpha antibody. Image from verified customer review.
	Flow (Intracellular): PGC1 alpha Antibody [NBP1-04676] - An intracellular stain was performed on HeLa cells with PGC1 alpha Antibody NBP1-04676AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

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Background

Named after its association with nuclear receptor peroxisome-proliferator activated receptor (PPAR) gamma, PGC-1 alpha (PPAR gamma coactivator 1-alpha) is now known to interact with a variety of transcription factors to regulate multiple aspects of cellular physiology. PGC-1 alpha binds MYBBP1A which results in inhibition of PGC-1 alpha's transcriptional activation potential and also interact with other proteins such as PRDM16, LRPPRC, LPIN1 etc. IRSs with core sequence T(G/A)TTTT serves as PGC-1 alpha's promoter and its transcription is repressed by ZNF746. CLK2 and AMPK execute PGC-1 alpha's phosphorylation and in the skeletal muscle its phosphorylation by AMPK increases activation of its own promoter. Under the condition of high nutrients, PGC-1 alpha is heavily acetylated by GCN5 resulting in its biological inactivation whereas it gets deacetylated by SIRT1 in low nutrients/high NAD conditions. PGC-1alpha acts as transcriptional coactivator for steroid and nuclear receptors, and enhances the transcriptional activity of PPARG as well as the thyroid hormone receptor on the uncoupling protein promoter. PGC-1alpha regulates key mitochondrial genes that contribute towards adaptive thermogenesis and plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism.

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