抗Histone H3 [p Ser28, Trimethyl Lys27]抗体 | Anti- Histone H3 [p Ser28, Trimethyl Lys27] antibody

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	Western Blot: Histone H3 [p Ser28, Trimethyl Lys27] Antibody [NB21-1193] - H3 K27Me3 antibody was tested in 1) HeLa histone prep 2) NIH-3T3 histone prep and 3) C. elegans embryo cell lysate.
	Immunocytochemistry/Immunofluorescence: Histone H3 [p Ser28, Trimethyl Lys27] Antibody [NB21-1193] - Immunofluorescence: [NB21-1193] - Histone H3 K27me3/pS28 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
	Chromatin Immunoprecipitation: Histone H3 [p Ser28, Trimethyl Lys27] Antibody [NB21-1193] - Chromatin from one million formaldehyde cross-linked HeLa cells was used with 2ug of NB21-1193 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.
	Immunocytochemistry/Immunofluorescence: Histone H3 [p Ser28, Trimethyl Lys27] Antibody [NB21-1193] - Immunofluorescence: [NB21-1193] - Histone H3 K27me3/pS28 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
	Histone H3 [p Ser28, Trimethyl Lys27] Antibody [NB21-1193]
	Dot Blot: Histone H3 [p Ser28, Trimethyl Lys27] Antibody [NB21-1193] - Dot Blot analysis of NB21-1193 using the peptides listed above in 100, 10, and 1 picomoles of peptide.

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Background

Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. Specifically, trimethylation of K27 is associated with gene silencing, whereas pS28 is associated with mitotis and immediate early genes.

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