抗TLR2 (TL2.1)抗体 | Anti- TLR2 (TL2.1) antibody

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	Flow Cytometry: TLR2 Antibody (TL2.1) - Azide Free [NB100-56726] - Analysis using Azide/BSA FREE version of NB100-56726. Cell surface flow analysis of TLR2 in 10^6 PBMCs using 1 ug of this antibody. Shaded histogram represents cells without antibody; green represents isotype control; red represents anti-TLR2 antibody.
	Flow Cytometry: TLR2 Antibody (TL2.1) - Azide Free [NB100-56726] - Intracellular analysis of TLR2 in 10^6 PBMs using 2 ug of TLR2 antibody. Shaded histogram represents cells without antibody; green represents isotype control ; red represents TLR2 antibody. goat anti-mouse IgG FITC secondary antibody was used in this test.
	Flow Cytometry: TLR2 Antibody (TL2.1) - Azide Free [NB100-56726] - Analysis using the FITC conjugate of NB100-56726. Staining of TLR2 in 10^6 PBMCs (lymphocyte-gated) using 2 ugs ofMouse IgG2a Kappa, and red represents TLR2 antibody.
	Flow Cytometry: TLR2 Antibody (TL2.1) - Azide Free [NB100-56726] - Analysis using the FITC conjugate of NB100-56726. Staining of TLR2 in 10^6 ThP1 cells using 2 ug of this antibody. The closed histogram represents cells alone, green represents isotype control, red represents anti-TLR2 antibody.
	Flow (Intracellular): TLR2 Antibody (TL2.1) - Azide Free [NB100-56726] - Analysis using the PE conjugate of NB100-56726. Staining of TLR2 on PBMC using this antibody at 1 ug/10^6 cells. Green represents isotype control this antibody; red represents anti-TLR2 antibody.
	Flow (Cell Surface): TLR2 Antibody (TL2.1) - Azide Free [NB100-56726] - Analysis using the FITC conjugate of NB100-56726. Surface staining of stable HEK293/hTLR2 cells (IML-202, red) and vector control cells (IML-200, green) using TLR2 antibody at 1 ug/10^6 cells.
	Flow (Cell Surface): TLR2 Antibody (TL2.1) - Azide Free [NB100-56726] - Analysis using the PE conjugate of NB100-56726. Staining of TLR2 on stable transfected cell line using this antibody at 1 ug/10^6 cells. Green represents isotype control; red represents anti-TLR2 antibody.
	Flow Cytometry: TLR2 Antibody (TL2.1) - Azide Free [NB100-56726] - Analysis using Azide/BSA FREE version of NB100-56726. Intracellular flow analysis of TLR2 in 1x10^6 PBMCs using 2 ug of NB100-56722. Shaded histogram represents cells without antibody; green represents isotype control (Sigma, Cat. No. M5409); purple represents anti-TLR2 antibody.

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Background

The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). TLR2 is differentially expressed in human cells. TLR2 is expressed in tonsils, lymph nodes, and appendices, activated B-cells in germinal centers. CD14+ monocytes expressed the highest level of TLR2 followed by CD15+ granulocytes, and CD19+ B-cells, CD3+ T-cells, and CD56+ NK cells did not express TLR2. The expression of TLR2 on different cell types are regulated by different immune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes (4).

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